You might want to take a look at the following paper: Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):479-83. How many water molecules can be detected by protein crystallography?
Carugo O<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Carugo%20O%22%5BAuthor%5D>, Bordo D<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Bordo%20D%22%5BAuthor%5D> . Mario Sanches On Wed, Feb 16, 2011 at 3:48 PM, Filip Van Petegem < filip.vanpete...@gmail.com> wrote: > Hello all, > > Some crystal structures seem to contain a very low number of water > molecules given their resolution (e.g 1 water for every 10 amino acid > residues at 2.2Angstrom, whereas probably around 0.6 waters per residue is > expected on average). I was wondering if anybody has any insights (or > better: a good reference) into the precise reasons. Of course general data > quality comes into mind, or using data-to-parameter ratio rather than > resolution. But how about intrinsic properties the protein? > > So my exact questions are: > - How frequent is a very low number of visible waters observed? > - What are the usual reasons? > > Cheers > > Filip Van Petegem > > -- > Filip Van Petegem, PhD > Assistant Professor > The University of British Columbia > Dept. of Biochemistry and Molecular Biology > 2350 Health Sciences Mall - Rm 2.356 > Vancouver, V6T 1Z3 > > phone: +1 604 827 4267 > email: filip.vanpete...@gmail.com > http://crg.ubc.ca/VanPetegem/ > -- Mario Sanches Postdoctoral Fellow Samuel Lunenfeld Research Institute Mount Sinai Hospital 600 University Ave Toronto - Ontario Canada M5G 1X5 http://ca.linkedin.com/in/mariosanches
