Hi Faisal, 3 - 3.5M Ammonium sulphate with your buffer should work too. Take a small loop.
Jan -- Jan Abendroth Emerald BioStructures Seattle / Bainbridge Island WA, USA home: Jan.Abendroth_at_gmail.com work: JAbendroth_at_embios.com http://www.emeraldbiostructures.com On May 23, 2013, at 2:42 AM, Faisal Tarique <faisaltari...@gmail.com> wrote: > > Dear all > > Can anybody tell me the appropriate cryo condition for the crystals obtained > in 2M Ammonium sulphate and tris pH8.5..I tried to add 10% glycerol to it but > still the ice ring is forming.. > > Thanx in advance > -- > Regards > > Faisal > School of Life Sciences > JNU
