The human branched-chain alpha-ketoacid dehydrogenase requires a K+ ion to stabilize the binding of the cofactor thiamine diphosphate and to achieve maximum catalytic efficiency. See
https://www.ncbi.nlm.nih.gov/pubmed/10745006 Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu<mailto:diana.tomch...@utsouthwestern.edu> (214) 645-6383 (phone) (214) 645-6353 (fax) On Jan 9, 2018, at 8:42 AM, Keller, Jacob <kell...@janelia.hhmi.org<mailto:kell...@janelia.hhmi.org>> wrote: Dear Crystallographers, Is anyone aware of a soluble protein which changes large-scale conformation +/- Na+, and is specific for Na+ per se, or at least ignores K+ and Ca++? E.g., Rb+ or Li+ might be okay. Structural info would be a plus, but not a sine qua non. Similarly, what about with K+ or Cl- specificities, but oblivious to similar common ions? All the best, Jacob Keller +++++++++++++++++++++++++++++++++++++++++++++++++ Jacob Pearson Keller Research Scientist / Looger Lab HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 (571)209-4000 x3159 +++++++++++++++++++++++++++++++++++++++++++++++++ The content of this email is confidential and intended for the recipient specified in message only. It is strictly forbidden to share any part of this message with any third party, without a written consent of the sender. If you received this message by mistake, please reply to this message and follow with its deletion, so that we can ensure such a mistake does not occur in the future. ________________________________ UT Southwestern Medical Center The future of medicine, today.
