Hi, I believe that one can put a 50-100uL drop of fresh SigmaCote (in a tube cap) with the glass pieces (surface well exposed), sealed in a dedicated (because the container will be coated too) container (air-tight lunch boxes). After a while the SigmaCote vapor should react with the glass and bring polysiloxane groups to the glass surface. I have done this with microscope slips. To make a few hundred .22 mm cover slips I think the major challenge is to make a frame for supporting the separated (~1mm apart to allow free diffusion?) cover slips (3D print?). Dry paper may work too. If some of the cover slips tend to stick together, a little vacuuming can help.
SigmaCote is a chlorinated polysiloxane. The Cl-Si group allows it to react with the hydroxyls on glass surface. Just be aware that the same group reacts even more readily with water in air, making the reagent less reactive with glass overtime (but I have used a VERY old bottle and it worked). References: https://en.wikipedia.org/wiki/Chlorosilane https://pubs.acs.org/doi/pdfplus/10.1021/j100727a046 https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/sl2pis.pdf Zhijie On 31/01/2019 4:16 a.m., herman.schreu...@sanofi.com<mailto:herman.schreu...@sanofi.com> wrote: A long time ago, before siliconized coverslips became commercially available, we used to siliconize coverslips ourselves. It is not really that much work and unsiliconized cover slips should be very cheap. If you wish, I could try to find back the protocol. Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Rajnandani Kashyap Gesendet: Donnerstag, 31. Januar 2019 09:17 An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Betreff: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? Dear All I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a normal siliconized coverslip and a 22mm siliconized circle coverslips. We tried to search for an alternative companies but couldn't get any one who sells coverslips with the same dimensions (0.19-0.22mm glass thickness and 22 mm glass diameter). Is there any alternative company (distribution in India) from where we can buy them for a reasonable price? Thanks in advance for sparing your valuable time and efforts. Regards Rajnandani Kashyap India ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=YlILrL-rg1OZBp9DSEAcOqrUs8BY0vFG_gPlaAB3qNk&s=t6ZqJh8ledXY32ENHFaeBd9vgbGKNUke1ROmyJzdM6k&e=> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
