Contour the 2Fo-Fc map at lower sigma than 1.3, and see if the resulting density looks like MES. As others have said, if there is no specific pocket or interactions for the non-sulfonic acid head group, then I would expect that density to be weaker or smeared out due to positional disorder.
Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry UT Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu<mailto:diana.tomch...@utsouthwestern.edu> (214) 645-6383 (phone) (214) 645-6353 (fax) On Mar 13, 2022, at 9:19 AM, Amir Khan <k...@crystal.harvard.edu<mailto:k...@crystal.harvard.edu>> wrote: EXTERNAL MAIL Hi,<tadpole.png> I wonder whether anyone can advise on the ‘tadpole’ like density. The head is currently a phosphate and I’ve placed a water in tail, but it obviously looks connected… would appreciate any help! Green density at 5 sigma (Fo-fc), while blue is 2fo-fc at 1.3 sigma. Crystallization condition is unknown, though it came from sparse matrix commercial (either Wizard, Pact Prem, or JCSG+). Thanks! Amir ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 CAUTION: This email originated from outside UTSW. Please be cautious of links or attachments, and validate the sender's email address before replying. ________________________________ UT Southwestern Medical Center The future of medicine, today. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
