Hi Manoj

If the structures are in the PDB, then PDB-KB may have already done the work 
for you - 

        https://www.ebi.ac.uk/pdbe/pdbe-kb/

They run Gesamt on homologous structures for the whole PDB every week (as I 
understand it).

Harry

> On 2 Mar 2023, at 07:45, Manoj N <nma...@iitm.ac.in> wrote:
> 
> Dear all,
>                 We want to generate a structure-based multiple sequence 
> alignment for a large number of homologous structures (>100). 
> 
> What are the available programs (stand-alone or webserver) to do this? 
> 
> Thank you,
> Best wishes,
> Manoj
> 
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