Hi Well worth contacting your favourite (or favorite…) synchrotron about in-plate data collection - I’d imagine that APS has something in-place (there was a report way back in the depths of time [2015, when I still worked in the field…] about a prototype on ID-19?), and that might be easier to get samples to (from Birmingham) than one of the European facilities.
Harry > On 23 Nov 2023, at 09:31, Jose A. MARQUEZ <marq...@embl.fr> wrote: > > Dear Elizabeth, > > In situ data collection is a good approach to try in your case. You could use > the CrystalDirect technology for automated crystal harvesting that is more > gentle to crystals than manual harvesting. This is available both at EMBL > Grenoble and EMBL Hamburg facilities, which offer integrated crystallography > services in collaboration with the ESRF and Petra III synchrotrons. > > doi:10.3791/62491. > doi:10.1016/j.crmeth.2021.100102. > doi:10.1107/s0907444912031459. > > Best wishes > > Josan > _________________________________________________________ > Jose A. Marquez, Senior Scientist > Head of the Crystallization Facility > European Molecular Biology Laboratory, Grenoble. > Delivery address: EMBL, 71, Avenue des Martyrs > 38000 Grenoble, France > Postal address: EMBL, 71, Avenue des Martyrs > CS 90181 38042 Grenoble Cedex 9, France > Phone +33 (0)476 20 74 25 > Fax. +33 (0)476 20 71 99 > > > https://www.embl.org/groups/marquez/ > > > https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/ > > > https://htxlab.embl.org/ > > _________________________________________________________ > > On 11/22/2023 5:44 PM, Blake, Morgan Elizabeth wrote: >> Hello! >> >> I am a PhD student working on a crystallography project to wrap up my >> dissertation research. I have purified a complex of two proteins, and I can >> consistently grow crystals in 10% PEG3350, 0.2M KSCN, 0.1M BIS-TRIS propane >> pH 7.5. These crystals have sharp edges and can grow to a large size >> (greater than 0.5 mm), but the crystals seem to be very fragile. When we >> open the drops to harvest the crystals, we have little time to harvest the >> crystals before they crack. When we move the crystals to a cryoprotectant, >> over time they start fracturing. We've tried using different percentages of >> glycerol, ethylene glycol, PEG400, and oil for cryoprotectants with no >> success. Needless to say, the crystals do not diffract well, with spot >> patterns that look very streaky/mosaic, which I presume is due to the >> defects that we see in harvesting/handling. We have screened for alternate >> crystallization conditions, but we seem to get the same morphology in other >> conditions. Does anyone have suggestions for additives we could use >> post-crystallization to help stabilize our crystals? >> >> Thanks for your advice! >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
