[ccp4bb] Second NYC Integrative Structural Biology symposium (NYC-ISB24) - Oct 9-11, 2024 - NYC, USA

[Ed Eng]

Dear all,

General registration is now open for NYC-ISB24:
 http://nyc-isb24.eventbrite.com

This 3-day event will be held October 9-11, 2024 at CUNY 
ASRC and NYSBC, and is 
focused on integrative structural biological techniques. Integrative structural 
biology is a powerful approach to understand biological macromolecular systems. 
By combining computational methods with structural science disciplines, spatial 
and temporal models of macromolecular targets in their in-situ context can be 
determined.

Invited Speakers include:

  *
Steve Bonilla (Rockefeller University)
  *
David Cowburn (Albert Einstein College of Medicine)
  *
Melinda Diver (Memorial Sloan Kettering Institute)
  *
Anum Glasgow (Columbia University)
  *
Eric Greene (Columbia University)
  *
Sonya Hanson (Flatiron Institute)
  *
Chi-Min Ho (Columbia University)
  *
Stephen Long (Memorial Sloan Kettering Institute)
  *
Michael Rudolph (New York Structural Biology Center)
  *
Ruth Stark (City University of New York)
  *
Ellen Zhong (Princeton University)
  *
Additional speakers will be chosen from abstracts.

We encourage you to submit an abstract for an oral presentation or poster by 
August 18, 2024.

See you in NYC this October.

Regards,
Ed on behalf of the #NYC-ISB24 organizing committee

Email: nyc-...@nysbc.org
Website: nysbc.org/nyc-isb24/

We thank our sponsors who have made this event possible: Thermo Fisher 
Scientific, Gatan/Ametek, Nanosoft, Quantum detectors, MiTeGen, Nanotemper, 
Nanoscience Instruments, Single Particle LLC, Schrödinger, AWS, SPTLabtech, 
Dectris and Angstrom Scientific, Inc.




From: Ed Eng
Sent: Tuesday, April 9, 2024 6:06 PM
To: cc...@jiscmail.ac.uk ; 3...@ncmir.ucsd.edu 
<3...@ncmir.ucsd.edu>; ccp4bb@jiscmail.ac.uk 
Cc: nyc-isb 
Subject: Save the date: NYC-ISB24 Integrative Structural Biology symposium - 
Oct 9-11, 2024 (NYC, USA)

Dear all,

Integrative structural biology is a powerful approach to understand biological 
macromolecular systems by bridging structural biological and biophysical tools. 
We are delighted to announce that the Advanced Science Research Center (ASRC) 
and New York Structural Biology Center (NYSBC) will host our 2nd NYC 
integrative structural biology symposium October 9-11, 2024.

For our program we have planned:

  1.
Wednesday, October 9th to be filled with workshops and hands-on demos, and
  2.
Thursday, October 10 and Friday, October 11 a symposium that dives into 
critical topics in the field.

More information will come soon. We are very much looking forward to welcoming 
you in NYC this fall.

Regards,
Ed Eng on behalf of the #NYC-ISB24 organizing committee
https://nysbc.org/nyc-isb24/

---
Edward T. Eng, Ph.D.
Senior Scientist, Manager
New York Structural Biology Center
89 Convent Ave, NY, NY 10027
nysbc.org | (212)939-0660 x344



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[ccp4bb] Postdoc position Sweden

[Magnus Andersson]

Hi,

We’re looking for a postdoc at Umeå University to work on membrane protein 
structural biology with a variety of methods, such as cryo-EM and X-ray 
solution scattering. The project can be accommodated to fit the interests of 
the candidate.

This is a great opportunity to contribute to developing membrane protein 
structural biology within the Integrated Structural Biology community at Umeå 
University.

https://www.nature.com/naturecareers/job/12817729/postdoctoral-scholarship-2-years-within-regulation-of-membrane-protein-transport/

Best,

Magnus

###
Magnus Andersson, Associate Professor
Integrated Structural Biology
Department of Chemistry
Umeå University

Email: magnus.p.anders...@umu.se
Phone: +46 720 837370

Mailing address:
Umeå University
Department of Chemistry
KBC-building
SE-901 87 Umeå

Visiting address:
Linnaeusvägen 6





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Re: [ccp4bb] Experimental phasing Selenomethionine data collection etc. tips

[Tom Peat]

I agree with Harry, I suspect that embarrassment has no definition/ meaning in 
the context of Nature.

From: CCP4 bulletin board  on behalf of Harry Powell 
<193323b1e616-dmarc-requ...@jiscmail.ac.uk>
Sent: Tuesday, May 14, 2024 6:28 PM
To: CCP4BB@JISCMAIL.AC.UK 
Subject: Re: [ccp4bb] Experimental phasing Selenomethionine data collection 
etc. tips

Hi

With this in mind, I have found the easiest way to generate AlphaFold models 
(for those not in the DB) is actually in CCP4 Cloud-remote - makes MR using AF 
models a real doddle.

See the Tutorials (in particular on MR) available with CCP4 Cloud.

Harry

> On 14 May 2024, at 09:20, Randy John Read  wrote:
>
> Dear Marco,
>
> You don’t mention here or in the earlier thread whether you tried AlphaFold 
> models and, if you did, how you prepared them for MR. I’m happy to hear of 
> any case where we still need experimental phasing methods to solve new 
> protein structures, but we’ve seen very few examples where AlphaFold models 
> didn’t work!
>
> I’m probably not the only one who would be delighted to take a look at the 
> problem to see what we can learn from it, if AlphaFold models really aren’t 
> working.
>
> Now, back to your question: to prepare for a SAD phasing experiments one 
> place I would look would be Tom Terwilliger’s recent papers on planning and 
> analysing SAD experiments (https://doi.org/10.1107/S2059798315019269, 
> https://doi.org/10.1107/S2059798315019403) as well as other information on 
> this from the Phenix website, including the YouTube tutorials.
>
> Best wishes,
>
> Randy Read
>
>> On 14 May 2024, at 01:17, Marco Bravo 
>>  wrote:
>>
>> Hello all,
>> I have a data collection trip next week and plan to collect data on 
>> selenomethionine derivative crystals at the al831 beamline. Are there any 
>> resources, tips, tutorials, literature etc. That you can recommend to help 
>> me prepare for these experiments. Also is there a way to plug in the 
>> experimental data into ccp4 cloud to do the automatic structure solution? Do 
>> I need native and derivative data to solve the structure? Last trip I 
>> collected a seemingly 2.8 angstrom resolution data on a crystal of the 
>> native protein but could not get a solution depsite extensive molecular 
>> replacement attempts. It seems that assigning a space group for the crystals 
>> has been troublesome as well. here is my last thread I posted about the 
>> issue for reference.
>>
>> https://www.jiscmail.ac.uk/cgi-bin/wa-jisc.exe?A2=ind2402=CCP4BB=D=CCE6DFA19FA3D40346=mbrav005%40ucr.edu=112302
>>
>> 
>>
>> To unsubscribe from the CCP4BB list, click the following link:
>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1
>>
>> This message was issued to members of http://www.jiscmail.ac.uk/CCP4BB, a 
>> mailing list hosted by http://www.jiscmail.ac.uk/, terms & conditions are 
>> available at https://www.jiscmail.ac.uk/policyandsecurity/
>
> -
> Randy J. Read
> Department of Haematology, University of Cambridge
> Cambridge Institute for Medical Research Tel: +44 1223 336500
> The Keith Peters Building
> Hills Road   E-mail: 
> rj...@cam.ac.uk
> Cambridge CB2 0XY, U.K.  
> www-structmed.cimr.cam.ac.uk
>
>
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
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Re: [ccp4bb] Experimental phasing Selenomethionine data collection etc. tips

[Orr, Christian (DLSLtd,RAL,LSCI)]

Hi Marco,

An alternative would be to scrap the SeMet crystals and collect anomalous data 
on native crystals at I23, Diamond Light Source. We are optimised for S-SAD and 
could likely help you with your phasing. Happy to talk off-list if you'd like 
to come and do an experiment (I am a beamline scientist on I23), just drop me 
and email (christian@diamond.ac.uk).
If you'd like to check the feasibility of the experiment, I made a web app 
where you put in a bit of info and it will tell you the likelihood of 
successful S-SAD phasing on I23 - https://diamondi23.anvil.app/.

Best,

Chris




From: CCP4 bulletin board  on behalf of dbellini 

Sent: Tuesday, May 14, 2024 06:23
To: CCP4BB@JISCMAIL.AC.UK 
Subject: Re: [ccp4bb] Experimental phasing Selenomethionine data collection 
etc. tips

[You don't often get email from dbell...@mrc-lmb.cam.ac.uk. Learn why this is 
important at https://aka.ms/LearnAboutSenderIdentification ]

Hi Marco,

A few suggestions that I like to follow for MAD experiments:

Before everything, check you have at least about 1 SeMet per 100
residues
Then before crystallisation check by MassSpec that SeMet is properly
incorporated in your protein
After crystallisation collect first on the peak with (very) high
redundancy and as little/gentle dose as possible
Collecting the other wavelengths should give you better starting
phases/maps, which might be very helpful at your resolution of 2.8
(especially if it is a very anisotropic 2.8...)

Automated pipelines are so good nowadays, if you collect good data they
should solve it without problems (as long as your crystal is not
suffering from other pathologies like twinning or pseudosymmtries).

Good luck!

D


On 2024-05-14 01:17, Marco Bravo wrote:
> CAUTION: This email originated from outside of the LMB:
> .-owner-ccp...@jiscmail.ac.uk-.
> Do not click links or open attachments unless you recognize the sender
> and know the content is safe.
> If you think this is a phishing email, please forward it to
> phish...@mrc-lmb.cam.ac.uk
> --
>
> Hello all,
> I have a data collection trip next week and plan to collect data on
> selenomethionine derivative crystals at the al831 beamline. Are there
> any resources, tips, tutorials, literature etc. That you can recommend
> to help me prepare for these experiments. Also is there a way to plug
> in the experimental data into ccp4 cloud to do the automatic structure
> solution? Do I need native and derivative data to solve the structure?
> Last trip I collected a seemingly 2.8 angstrom resolution data on a
> crystal of the native protein but could not get a solution depsite
> extensive molecular replacement attempts. It seems that assigning a
> space group for the crystals has been troublesome as well. here is my
> last thread I posted about the issue for reference.
>
> https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwa-jisc.exe%3FA2%3Dind2402%26L%3DCCP4BB%26O%3DD%26X%3DCCE6DFA19FA3D40346%26Y%3Dmbrav005%2540ucr.edu%26P%3D112302=05%7C02%7Cchristian.orr%40DIAMOND.AC.UK%7Cc6bee2ad846744e1ab3208dc73d65bde%7C9d27ba7401004d0d81ff1d728dae8df6%7C0%7C0%7C638512612017758737%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C=WQBkVO%2Frfrm3B9WGhe%2BB5CnOvevahEm7C7xyjoEkGak%3D=0
>
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
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>  a
> mailing list hosted by 
> 

Re: [ccp4bb] AlphaFold3 Transparency and Reproducibility

[Harry Powell]

Do Nature do embarrassment?

H

> On 14 May 2024, at 10:54, Frank von Delft 
>  wrote:
> 
> What we really need is a tweet from Nature, declaring their embarrassment at 
> having been suckered into becoming DeepMind's advertising arm.  
> 
> 
> On 14/05/2024 01:56, Paul Adams wrote:
>> 
>> The letter may have had (or helped have) an impact already:
>> 
>> On X today from Pushmeet Kohli @ DeepMind
>> 
>> "We love the excitement & results from the community on AlphaFold 3 and are 
>> doubling the AF Server daily job limit to 20. Happy to also share that we're 
>> working on releasing the AF3 model (incl weights) for academic use, which 
>> doesn’t depend on our research infra, within 6 months".
>> 
>> 
>>> On May 13, 2024, at 11:11 AM, Wankowicz, Stephanie 
>>>  wrote:
>>> 
>>> If it does what it claims – incredibly impressive. The issue we have is 
>>> there is no way to verify or validate the claims it is making. 
>>> 
>>> The letter is a call and start of a conversation about how the 
>>> ever-changing landscape of communication and publication of science should 
>>> be.
>>> 
>>> -Stephanie Wankowicz
>>> From: CCP4 bulletin board  on behalf of Krieger, 
>>> James M 
>>> Sent: Monday, May 13, 2024 10:22 AM
>>> To: CCP4BB@JISCMAIL.AC.UK 
>>> Subject: Re: [ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility
>>>  
>>> This Message Is From an External Sender 
>>> This message came from outside your organization. 
>>> It definitely is impressive but it also has clear limitations
>>> 
 On 13 May 2024, at 15:22, Sylvia Fanucchi 
  wrote:
 
 
 
 You don't often get email from 
 d0c4e77ae410-dmarc-requ...@jiscmail.ac.uk. Learn why this is important
 
 Is it just me who is really impressed by it? Am I missing something?
 
 Get Outlook for Android
 From: CCP4 bulletin board  on behalf of Rafael 
 Marques 
 Sent: Monday, May 13, 2024 3:13:16 PM
 To: CCP4BB@JISCMAIL.AC.UK 
 Subject: Re: [ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility
  
 I tried it yesterday and I was really shocked by how fast it is. When I 
 was preparing to submit my second job, the first one was already finished, 
 which made me think that I was definitely doing something wrong. Probably 
 I was...
 
 Best wishes 
 
 Rafael Marques 
 
 
 On 13 May 2024 09:53, Harry Powell 
 <193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote:
 Hi folks 
 
 This arrived in my inbox this morning, and I believe that it may provoke 
 some discussion…
 
 Wikipedia tells me: στέργει γὰρ οὐδεὶς ἄγγελον κακῶν ἐπῶν 
 
 Best wishes! 
 
 Harry 
 
 >From: Stephanie Wankowicz  
 >Sent: Saturday, May 11, 2024 3:31 PM 
 >To: James Fraser ; Pedro Beltrao 
 > ; Benjamin Cravatt ; Roland 
 > Dunbrack ; Anthony Gitter 
 > ; Kresten Lindorff-Larsen ; 
 > Sergey Ovchinnikov ; Polizzi, Nicholas F. 
 > ; Brian Shoichet 
 > 
 >Subject: AlphaFold3 Transparency and Reproducibility 
 > 
 > 
 >This email from mullane.stepha...@gmail.com originates from outside 
 > Imperial. Do not click on links and attachments unless you recognise the 
 > sender. If you trust the sender, add them to your safe senders list 
 >  to disable email 
 > stamping for this address. 
 > 
 >Hello, 
 > 
 > 
 >As many of you, we were incredibly disappointed with the lack of code 
 > or even executables accompanying the publication of AlphaFold3 in 
 > Nature. AlphaFold3 was released without the means to test and use the 
 > software in a high-throughput manner. This does not align with the 
 > principles of scientific research, which rely on the ability of the 
 > community to evaluate, use, and build upon existing work.
 > 
 > 
 > 
 >We have written a letter, which will be posted on Zenodo and 
 > submitted as a Letter to the Editor in the coming days.
 > 
 > 
 > 
 >Please see the entire letter here. 
 > 
 >  If you want to endorse this letter, please fill out your name, 
 > affiliation, and email in the form.
 > 
 > 
 > 
 >Additionally, a PDF version of the letter can be found here 
 > .
 > 
 > 
 > 
 >Thank you, 
 > 
 > 
 > 
 >Stephanie Wankowicz, UCSF 
 > 
 >Pedro Beltrao, ETH 
 >Benjamin Cravatt, Scripps 
 >Roland Dunbrack, FCCC 
 >Anthony Gitter, UW Madison 
 >

Re: [ccp4bb] AlphaFold3 Transparency and Reproducibility

[Frank von Delft]

What we really need is a tweet from Nature, declaring their 
embarrassment at having been suckered into becoming DeepMind's 
advertising arm.



On 14/05/2024 01:56, Paul Adams wrote:


The letter may have had (or helped have) an impact already:

On X today from Pushmeet Kohli @ DeepMind

"We love the excitement & results from the community on AlphaFold 3 
and are doubling the AF Server daily job limit to 20. Happy to also 
share that we're working on releasing the AF3 model (incl weights) for 
academic use, which doesn’t depend on our research infra, within 6 
months".



On May 13, 2024, at 11:11 AM, Wankowicz, Stephanie 
 wrote:


If it does what it claims – incredibly impressive. The issue we have 
is there is no way to verify or validate the claims it is making.


The letter is a call and start of a conversation about how the 
ever-changing landscape of communication and publication of science 
should be.


-Stephanie Wankowicz

*From:*CCP4 bulletin board  on behalf of 
Krieger, James M 

*Sent:*Monday, May 13, 2024 10:22 AM
*To:*CCP4BB@JISCMAIL.AC.UK
*Subject:*Re: [ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility
This Message Is From an External Sender
This message came from outside your organization.
It definitely is impressive but it also has clear limitations

On 13 May 2024, at 15:22, Sylvia Fanucchi 
 wrote:





You don't often get email from 
d0c4e77ae410-dmarc-requ...@jiscmail.ac.uk.Learn why this is 
important 




Is it just me who is really impressed by it? Am I missing something?

GetOutlook for Android 



*From:*CCP4 bulletin board  on behalf of 
Rafael Marques 

*Sent:*Monday, May 13, 2024 3:13:16 PM
*To:*CCP4BB@JISCMAIL.AC.UK 
*Subject:*Re: [ccp4bb] Fwd: AlphaFold3 Transparency and Reproducibility
I tried it yesterday and I was really shocked by how fast it is. 
When I was preparing to submit my second job, the first one was 
already finished, which made me think that I was definitely doing 
something wrong. Probably I was...


Best wishes

Rafael Marques


On 13 May 2024 09:53, Harry Powell 
<193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote:


Hi folks

This arrived in my inbox this morning, and I believe that it may
provoke some discussion…

Wikipedia tells me: στέργει γὰρ οὐδεὶς ἄγγελον κακῶν ἐπῶν

Best wishes!

Harry

>    From: Stephanie Wankowicz 
>    Sent: Saturday, May 11, 2024 3:31 PM
>    To: James Fraser ; Pedro Beltrao
; Benjamin Cravatt
; Roland Dunbrack
; Anthony Gitter
; Kresten Lindorff-Larsen
; Sergey Ovchinnikov ; Polizzi,
Nicholas F. ; Brian Shoichet

>    Subject: AlphaFold3 Transparency and Reproducibility
>
>
>    This email from mullane.stepha...@gmail.com originates from
outside Imperial. Do not click on links and attachments unless
you recognise the sender. If you trust the sender, add them to
your safe senders list


to
disable email stamping for this address.
>
>    Hello,
>
>
>    As many of you, we were incredibly disappointed with the
lack of code or even executables accompanying the publication of
AlphaFold3 in Nature. AlphaFold3 was released without the means
to test and use the software in a high-throughput manner. This
does not align with the principles of scientific research, which
rely on the ability of the community to evaluate, use, and build
upon existing work.
>
>
>
>    We have written a letter, which will be posted on Zenodo
and submitted as a Letter to the Editor in the coming days.
>
>
>
>    Please see the entire letter here.



If
you want to endorse this letter, please fill out your name,
affiliation, and email in the form.
>
>
>
>    

Re: [ccp4bb] Experimental phasing Selenomethionine data collection etc. tips

[Harry Powell]

Hi

With this in mind, I have found the easiest way to generate AlphaFold models 
(for those not in the DB) is actually in CCP4 Cloud-remote - makes MR using AF 
models a real doddle. 

See the Tutorials (in particular on MR) available with CCP4 Cloud.

Harry

> On 14 May 2024, at 09:20, Randy John Read  wrote:
> 
> Dear Marco,
> 
> You don’t mention here or in the earlier thread whether you tried AlphaFold 
> models and, if you did, how you prepared them for MR. I’m happy to hear of 
> any case where we still need experimental phasing methods to solve new 
> protein structures, but we’ve seen very few examples where AlphaFold models 
> didn’t work!
> 
> I’m probably not the only one who would be delighted to take a look at the 
> problem to see what we can learn from it, if AlphaFold models really aren’t 
> working.
> 
> Now, back to your question: to prepare for a SAD phasing experiments one 
> place I would look would be Tom Terwilliger’s recent papers on planning and 
> analysing SAD experiments (https://doi.org/10.1107/S2059798315019269, 
> https://doi.org/10.1107/S2059798315019403) as well as other information on 
> this from the Phenix website, including the YouTube tutorials.
> 
> Best wishes,
> 
> Randy Read
> 
>> On 14 May 2024, at 01:17, Marco Bravo 
>>  wrote:
>> 
>> Hello all,
>> I have a data collection trip next week and plan to collect data on 
>> selenomethionine derivative crystals at the al831 beamline. Are there any 
>> resources, tips, tutorials, literature etc. That you can recommend to help 
>> me prepare for these experiments. Also is there a way to plug in the 
>> experimental data into ccp4 cloud to do the automatic structure solution? Do 
>> I need native and derivative data to solve the structure? Last trip I 
>> collected a seemingly 2.8 angstrom resolution data on a crystal of the 
>> native protein but could not get a solution depsite extensive molecular 
>> replacement attempts. It seems that assigning a space group for the crystals 
>> has been troublesome as well. here is my last thread I posted about the 
>> issue for reference.
>> 
>> https://www.jiscmail.ac.uk/cgi-bin/wa-jisc.exe?A2=ind2402=CCP4BB=D=CCE6DFA19FA3D40346=mbrav005%40ucr.edu=112302
>> 
>> 
>> 
>> To unsubscribe from the CCP4BB list, click the following link:
>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1
>> 
>> This message was issued to members of http://www.jiscmail.ac.uk/CCP4BB, a 
>> mailing list hosted by http://www.jiscmail.ac.uk/, terms & conditions are 
>> available at https://www.jiscmail.ac.uk/policyandsecurity/
> 
> -
> Randy J. Read
> Department of Haematology, University of Cambridge
> Cambridge Institute for Medical Research Tel: +44 1223 336500
> The Keith Peters Building
> Hills Road   E-mail: 
> rj...@cam.ac.uk
> Cambridge CB2 0XY, U.K.  
> www-structmed.cimr.cam.ac.uk
> 
> 
> 
> 
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1
> 
> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing 
> list hosted by www.jiscmail.ac.uk, terms & conditions are available at 
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Re: [ccp4bb] Experimental phasing Selenomethionine data collection etc. tips

[Randy John Read]

Dear Marco,

You don’t mention here or in the earlier thread whether you tried AlphaFold 
models and, if you did, how you prepared them for MR. I’m happy to hear of any 
case where we still need experimental phasing methods to solve new protein 
structures, but we’ve seen very few examples where AlphaFold models didn’t work!

I’m probably not the only one who would be delighted to take a look at the 
problem to see what we can learn from it, if AlphaFold models really aren’t 
working.

Now, back to your question: to prepare for a SAD phasing experiments one place 
I would look would be Tom Terwilliger’s recent papers on planning and analysing 
SAD experiments (https://doi.org/10.1107/S2059798315019269, 
https://doi.org/10.1107/S2059798315019403) as well as other information on this 
from the Phenix website, including the YouTube tutorials.

Best wishes,

Randy Read

> On 14 May 2024, at 01:17, Marco Bravo 
>  wrote:
>
> Hello all,
> I have a data collection trip next week and plan to collect data on 
> selenomethionine derivative crystals at the al831 beamline. Are there any 
> resources, tips, tutorials, literature etc. That you can recommend to help me 
> prepare for these experiments. Also is there a way to plug in the 
> experimental data into ccp4 cloud to do the automatic structure solution? Do 
> I need native and derivative data to solve the structure? Last trip I 
> collected a seemingly 2.8 angstrom resolution data on a crystal of the native 
> protein but could not get a solution depsite extensive molecular replacement 
> attempts. It seems that assigning a space group for the crystals has been 
> troublesome as well. here is my last thread I posted about the issue for 
> reference.
>
> https://www.jiscmail.ac.uk/cgi-bin/wa-jisc.exe?A2=ind2402=CCP4BB=D=CCE6DFA19FA3D40346=mbrav005%40ucr.edu=112302
>
> 
>
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-
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research Tel: +44 1223 336500
The Keith Peters Building
Hills Road   E-mail: 
rj...@cam.ac.uk
Cambridge CB2 0XY, U.K.  
www-structmed.cimr.cam.ac.uk




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[ccp4bb] PDRA in serial crystallography at Diamond Light Source

[Owen, Robin (DLSLtd,RAL,LSCI)]

Hi all,

We have a PDRA position open at Diamond to help us develop new methods for 
time-resolved crystallography. You will work closely with a number of groups at 
Diamond and beyond to improve approaches for both SSX and SFX.

For further info and to apply see
https://vacancies.diamond.ac.uk/vacancy/pdra-serial-and-microfocus-macromolecular-crystallography-554610.html
Please feel free to contact me if you have any questions.

Apologies but the deadline is soon: 19/5..
All the best,
Robin




Dr Robin Owen
Principal Beamline Scientist
I24, Microfocus Macromolecular Crystallography
Diamond Light Source, UK
Tel: +44 1235 778522
http://www.diamond.ac.uk/Beamlines/Mx/I24.html

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