Re: [ccp4bb] Assistant Professor in Structural Biology, University of Nebraska-Lincoln

Professor. Best regards, Mark Mark A. Wilson (he/him) Professor Department of Biochemistry/Redox Biology Center University of Nebraska N118 Beadle Center 1901 Vine St. Lincoln, NE 68588 From: Gerlind Sulzenbacher Date: Sunday, October 15, 2023 at 10:15 AM To: Mark Wilson , CCP4BB@JISCMAIL.AC.UK

[ccp4bb] Assistant Professor in Structural Biology, University of Nebraska-Lincoln

Dear Colleagues, The Department of Biochemistry at the University of Nebraska-Lincoln (UNL) invites applications for a tenure-track nine-month (academic year) faculty position at the rank of Assistant Professor. Areas of particular interest include but are not limited to time-resolved

[ccp4bb] Session on mix-and-inject serial crystallography at the SSRL/LCLS Users' Meeting

Dear fellow structural biologists, Dr. Chris Kupitz (LCLS) and I are co-hosting a session on mix-and-inject serial crystallography at XFELS and synchrotrons on Weds, 9/28 as part of this year’s SSRL/LCLS Users’ Meeting. This is an opportunity to hear from leading researchers about new methods

[ccp4bb] Cryo-EM Core Director position, University of Nebraska-Lincoln

Dear Structural Biologists, The University of Nebraska is hiring a Core Director (Research Assistant Professor) for our Glacios TEM with a Selectris energy filter, Falcon 4i detector, and micro-ED capability (delivery expected 12/2022). This hire is an addition to a tenure-leading hire in

[tianocore/edk2] b17080: UefiCpuPkg: Clean up save state boundary checks an...

Branch: refs/heads/master Home: https://github.com/tianocore/edk2 Commit: b170806518c1e414939c8b085866544814e1ce8e https://github.com/tianocore/edk2/commit/b170806518c1e414939c8b085866544814e1ce8e Author: Mark Wilson Date: 2021-09-01 (Wed, 01 Sep 2021) Changed paths: M

[ccp4bb] Fixing input anisotropic scale parameters in Refmac5

Dear Colleagues, I would like to run test refinements in Refmac5 where I can provide anisotropic scale parameters, fix them, and then proceed with restrained refinement of other model parameters. I could not find how to do this in the documentation-is this possible? Best regards, Mark Mark

[jira] [Created] (ARTEMIS-3088) How may Cash.app/help to make moment exchanges?

mark wilson created ARTEMIS-3088: Summary: How may Cash.app/help to make moment exchanges? Key: ARTEMIS-3088 URL: https://issues.apache.org/jira/browse/ARTEMIS-3088 Project: ActiveMQ Artemis

[jira] [Created] (ARTEMIS-3087) How might we get Cash app customer service to destroy a record?

mark wilson created ARTEMIS-3087: Summary: How might we get Cash app customer service to destroy a record? Key: ARTEMIS-3087 URL: https://issues.apache.org/jira/browse/ARTEMIS-3087 Project: ActiveMQ

Reminder - Next meeting of the GSE UK Security Working Group - 4th Feb 2021

ons around the 1st February. Regards Mark Wilson and Jamie Pease Jamie Pease CISA, CISM, CISSP, MBCS CITP, CDPSE Chairman of the GSE UK Security Working Group Website: https://www.gse.org.uk/working-group/enterprise-security/ Email: jamie.pe...@gse.org.uk<mailto:jamie.pe...@gse.org.uk> Mobi

Next meeting of the GSE UK Security Working Group - 4th Feb 2021

fer the opportunity to earn up to 8 CPE/CPD hours for attending the meeting. Please book your space asap if you wish to attend, as we have a limited number of spaces available! Regards Mark Wilson and Jamie Pease -- For IBM-M

Final Reminder: Next GSE UK Security Meeting - 11th June 2020 via Webex

ginning of next week. Stay safe and well. Regards Mark Wilson and Jamie Pease Jamie Pease CISA, CISM, CISSP, MBCS CITP Chairman of the GSE UK Security Working Group Website: https://www.gse.org.uk/working-group/enterprise-security/ Email: jamie.pe...@gse.org.uk<mailto:jamie.pe...@gse.org.

Reminder: Next GSE UK Security Meeting - 11th June 2020 via Webex

ay safe and well. Regards Mark Wilson and Jamie Pease Jamie Pease CISA, CISM, CISSP, MBCS CITP Chairman of the GSE UK Security Working Group Website: https://www.gse.org.uk/working-group/enterprise-security/ Email: jamie.pe...@gse.org.uk<mailto:jamie.pe...@gse.org.uk> Mobile: +44(

Next GSE UK Security Meeting - 11th June 2020 via Webex

nd well. Regards Mark Wilson and Jamie Pease Jamie Pease CISA, CISM, CISSP, MBCS CITP Chairman of the GSE UK Security Working Group Website: https://www.gse.org.uk/working-group/enterprise-security/ Email: jamie.pe...@gse.org.uk<mailto:jamie.pe...@gse.org.uk> Mobile: +44(

Important Update - Next meeting of the GSE UK Security Working Group

with registration details will be published over the coming weeks. In the meantime, please stay tuned to your mailbox or our website (https://www.gse.org.uk/working-group/enterprise-security/) for updates. Stay safe and well! Regards Mark Wilson and Jamie Pease Jamie Pease CISA, CISM, CISSP

Re: [ccp4bb] Covalent Cysteine Aduct

Hi Richard, Something that hasn’t been mentioned yet that might help settle the issue is to calculate an anomalous difference map with your I+, I- unmerged data. Even if you collect data far from the S edge, it is often possible to see positive anomalous difference density for S atoms in maps at

Save the date - Next meeting of the GSE UK Security Working Group

or our website (https://www.gse.org.uk/working-group/enterprise-security/) for updates. Regards Mark Wilson and Jamie Pease Jamie Pease CISA, CISM, CISSP, MBCS CITP Chairman of the GSE UK Security Working Group Website: www.racf.gse.org.uk<http://www.racf.gse.org.uk> Email: ja

Final Reminder - Next meeting of the GSE UK Security Working Group - 6th February 2020

red, you will receive joining instructions early next week. Regards Mark Wilson and Jamie Pease -- For IBM-MAIN subscribe / signoff / archive access instructions, send email to lists...@listserv.ua.edu with the message: INFO IBM-MAIN

Reminder - Next meeting of the GSE UK Security Working Group - 6th February 2020

ent to cater for a variety of professionals. We also offer the opportunity to earn CPE points for attending the meeting for those of you who are required to maintain professional certifications. We hope that you will be able to join us for the day. Regards Mark Wilson and Jamie Pe

Next meeting of the GSE UK Security Working Group - 6th February 2020

ure of technical and non-technical content to cater for a variety of professionals. We also offer the opportunity to earn CPE points for attending the meeting for those of you who are required to maintain professional certifications. We hope that you will be able to join us for the day. Regards Mark Wil

JES3 to JES2 migration experience

Varun, We have lots of migration experience. If you would like to discuss the gotcha’s for such a migration, drop me an email to ma...@rsmpartners.com Mark From: IBM Mainframe Discussion List mailto:IBM-MAIN@LISTSERV.UA.EDU>> On Behalf Of Mainframe Sysprog

Next meeting of the GSE UK Security Working Group - 6th Feb 2020

Ladies and Gentlemen, A date for your calendar! We are pleased to announce that the next meeting of the GSE UK Security Working Group, will take place on Thursday 6th February 2020 at the offices of SAS in Bishopsgate, Central London. Registration is now open, which you can access via our

[ccp4bb] Postdoctoral position in molecular image analysis methods development, University of Nebraska

Dear Colleagues, There is a postdoctoral position at the University of Nebraska in methods development for molecular image analysis. Application review has started and will remain open until the position is filled. Please see details below. Best regards, Mark Mark A. Wilson Associate Professor

Final reminder - Next meeting of the GSE UK Security Working Group

ave already registered, you will receive joining instructions by the end of this week. We hope that you will be able to join us either in person or virtually! Regards Mark Wilson and Jamie Pease -- For IBM-MAIN subscribe / sign

Reminder - Next meeting of the GSE UK Security Working Group

up to 7 CPE hours for attending this event. We hope that you will be able to join us either in person or virtually! Regards Mark Wilson and Jamie Pease -- For IBM-MAIN subscribe / signoff / archive access instructions, send em

Next meeting of the GSE UK Security Working Group - 6th June

ave a certification or you are studying, you can earn up to 7 CPEs for attending this event. We hope that you will be able to join us! Regards Mark Wilson and Jamie Pease Jamie Pease CISA, CISM, CISSP, MBCS CITP Chairman of the GSE UK Security Working Group Website: www.racf.gse.org.uk&l

Save the date - Next meeting of the GSE UK Security Working Group

, which you can access via our Events page > http://www.racf.gse.org.uk/content/content_events.php The agenda is currently being finalised and will be published at the end of March. Please save the date in your calendar and keep an eye on your mailbox for further updates from us. Regards M

Re: [ccp4bb] [EXTERNAL] [ccp4bb] refmac same residue different names

Hi Diana, At the risk of thread-jacking Ed’s question, yes, PHENIX handles this seamlessly for us as well. However, REFMAC5 doesn’t like this scenario, with or without alt. confs. or insertion codes. There are some models where I’d really like to use REFMAC5 for refining these mixed species

Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] refmac same residue different names

I agree. We have encountered refinement problems similar to those Ed describes for models with mixtures of modified cysteine species, which is a fairly common occurrence. Best regards, Mark Mark A. Wilson Associate Professor Department of Biochemistry/Redox Biology Center University of Nebraska

UK Based GenZ Networking Event on 22nd March 2019

For any younger members of RACF-L and IBMMAIN…. All are welcome, but the event will be held in Bromsgrove, UK… Mark Hi all, Following on from our successful launch at GSE last year, join us on March 22nd 2019, where the first GenZ full day event will take place at RSM Partners, Bromsgrove.

Reminder - Next meeting of the GSE UK Security Working Group - 7th February

?id=23 If you have a certification or you are studying, you can earn up to 7 CPEs for attending this event. We hope that you will be able to join us! Regards Mark Wilson and Jamie Pease Jamie Pease CISA, CISM, CISSP, MBCS CITP Chairman of the GSE UK Security Working Group W

Next meeting of the GSE UK Security Working Group - 7th February

?id=23 If you have a certification or you are studying, you can earn up to 7 CPEs for attending this event. We hope that you will be able to join us! Regards Mark Wilson and Jamie Pease -- For IBM-MAIN subscribe / signoff / a

Save the date - Next meeting of the GSE UK Security Working Group

ink will be of interest to the group, please send an e-mail to the Chairman (jamie.pe...@gse.org.uk<mailto:jamie.pe...@gse.org.uk>) with your bio and session abstract. Please save the date in your calendar and keep an eye on your mailbox for further updates from us. Regards Mark Wilson and Ja

Re: [ccp4bb] collective term for hydrogen bonds and salt bridges

Just to put a finer point on some of this, the Lennard-Jones (6-12) potential is short-range, containing an attractive component arising from London dispersion forces (quantum mechanical induced dipole-induced dipole interactions) that decay as 1/r^6 and a repulsive component arising from the

Reminder - Next meeting of the GSE UK Security Working Group - 28th June 2018

E hours for attending. We hope that you will be able to join us for another highly valuable GSE meeting! Regards Jamie Pease and Mark Wilson -- For IBM-MAIN subscribe / signoff / archive access instructions, send email t

Reminder - Next meeting of the GSE UK Security Working Group - 28th June 2018

E hours for attending. We hope that you will be able to join us for another highly valuable GSE meeting! Regards Jamie Pease and Mark Wilson -- For IBM-MAIN subscribe / signoff / archive access instructions, send email t

GSE UK Annual Conference

All, The vendor invite letter and call for papers PDFs have been loaded onto the GSE UK Conference website. Please take a look and let me know if you would like to participate. http://conferences.gse.org.uk/vsinfo Mark GSE Information Large Systems Working Group Chairman www.lsx.gse.org.uk

UK: Annual GSE Conference Call for papers

Hi, The call for papers for this year’s GSE UK conference has been issued and can be seen here: http://conferences.gse.org.uk/2018/call General conference information can also be seen here: http://conferences.gse.org.uk/2018 Mark GSE UK Conference Manager www.gse.org.uk/tyc Email:

Next meeting of the GSE UK Security Working Group - 28th June 2018

another highly valuable GSE meeting! Regards Jamie Pease and Mark Wilson -- For IBM-MAIN subscribe / signoff / archive access instructions, send email to lists...@listserv.ua.edu with the message: INFO IBM-MAIN

Reminder: Next meeting of the GSE UK Security Working Group - 8th February 2018

une at the offices of SAS UK in Marlow. Be sure to save the date in your calendar - registration will open in early March. More details are available via the link above. See you at the next meeting! Regards Jamie Pease and Mark Wil

OS/390 2.5

Hi Folks, I have a client who is running OS/390 2.5 and they cannot upgrade their OS for many reasons, let’s just not go there! They have asked what is the latest z Hardware they can use whilst running OS/390 2.5, but I am struggling to find any info out there at the moment. I know its old,

Re: [ccp4bb] [ccp4bb] protein precipitation reg

I heartily concur with Craig. Tris can be a dangerous buffer for many reasons, including those listed below. In addition, as a primary amine, it can complicate work with metalloproteins and has moderate nucleophilicity. There is almost always a better buffer choice than Tris. Best regards, Mark

IEFOPZxx Parmlib Members -- Debate at GSE LSG Meeting

on this over the coming week and may come up with more questions than answers! Hope this helps…. Mark Mark Wilson | Technical Director | RSM Partners Ltd Head Office: +44 (0) 1527 837767 Mobile: +44 (0) 7768 617006 Email:ma...@rsmpartners.com<mailto:ma...@rsmpartners.

[qubes-users] Save session Fedora-23 AppVM

Is possible to save session on Fedora-23 AppVM? (In a similar way as dom0.) Regards -- You received this message because you are subscribed to the Google Groups "qubes-users" group. To unsubscribe from this group and stop receiving emails from it, send an email to

PSF Issue

Hi, We’re trying to print Royal Mail MailMark barcodes which require C40 encoding throughout. When we have sent samples to Royal Mail they are being rejected because the c40 Latchcode is in the middle. Based on my reading this suggests that we are still using default encoding and not C40

Outsourcing Stories Good or Bad!

I am working with a client in Europe that is being requested by his senior management team to look at outsourcing their IT systems, including their system z platform. Would anyone be willing to share any war stories of their experiences with Outsourcing good or bad? Offline from the list via

Re: [ccp4bb] clash crash

I second Bernhard’s frustration with this, and have dealt with this many times. I note that SHELX uses an occupancy-based clash criterion that will not generate a clash for atoms whose occupancies sum to <1.1. It seems to me that this could be a more generally adopted criterion. Furthermore, it

One for the UK folks out there --- The next meeting of the GSE UK Security Working Group will take place on Thursday 4th February......

doesn't have to be a presentation - perhaps you would prefer to run an interactive group discussion on a pressing topic! Hope to see you all in 2016…Happy Christmas and a very Happy New Year… Regards Jamie Pease and Mark Wilson --

[Bug 1497393] [NEW] openvswitch-datapath-dkms 1.4.6-0ubuntu1.12.04.5: openvswitch kernel module failed to build

Public bug reported: This appears at every update ProblemType: Package DistroRelease: Ubuntu 12.04 Package: openvswitch-datapath-dkms 1.4.6-0ubuntu1.12.04.5 ProcVersionSignature: Ubuntu 3.13.0-63.104~precise1-generic 3.13.11-ckt25 Uname: Linux 3.13.0-63-generic i686 NonfreeKernelModules: nvidia

[Bug 1497393] [NEW] openvswitch-datapath-dkms 1.4.6-0ubuntu1.12.04.5: openvswitch kernel module failed to build

Public bug reported: This appears at every update ProblemType: Package DistroRelease: Ubuntu 12.04 Package: openvswitch-datapath-dkms 1.4.6-0ubuntu1.12.04.5 ProcVersionSignature: Ubuntu 3.13.0-63.104~precise1-generic 3.13.11-ckt25 Uname: Linux 3.13.0-63-generic i686 NonfreeKernelModules: nvidia

GSE UK - Large Systems Virtual Event

All, I am pleased to announce the agenda for the Virtual GSE UK - Large Systems Event. Full details can be seen here: http://lsx.gse.org.uk/content/content_news.php Mark -- For IBM-MAIN subscribe / signoff / archive access

Re: [ccp4bb] AW: [ccp4bb] Translational NCS with one molecule in ASU

ider statistical disorder, which will not show up in >twinning statistics since in this case F's (including phases) are added >instead of I's. Anyways, it will be an interesting puzzle to solve! > >Good luck, >Herman > > >-Ursprüngliche Nachricht- >Von: CCP4 bulletin

Re: [ccp4bb] Translational NCS with one molecule in ASU

t. So, I think, the >presence of twining can't be simply overruled in this case. reprocessing >the data in monoclinic SG, followed by doing > MR could be the key to this problem. > > >Good luck..!!! >Sudipta > > >On Wed, Sep 2, 2015 at 6:05 PM, Mark Wilson ><mwilso...@unl

Re: [ccp4bb] AW: [ccp4bb] Translational NCS with one molecule in ASU

essing >>>> coincidence somewhere is approaches 100%! >>>> >>>> What I would do in addition to excellent suggestions you already >>>> got, is >>>> to try to download the Fobs from the pdb for the structures with the >>&g

Re: [ccp4bb] AW: [ccp4bb] Translational NCS with one molecule in ASU

h the same SG is >yours bigger? > > > > >On 3 September 2015 at 18:56, Mark Wilson ><mwilso...@unl.edu> wrote: > >Dear Remy, >Indeed, I think you may be correct and we're pursuing this now. A perfect >0.5 lattice translocation along b in P212121 would (I thi

Re: [ccp4bb] AW: [ccp4bb] Translational NCS with one molecule in ASU

> >This would be my feeling too - one real 21, a twin axis and >pseudosymmetry. The standard perfect storm. > >Sent from my iPhone > >> On 3 Sep 2015, at 21:07, Mark Wilson <mwilso...@unl.edu> wrote: >> >> Hi Eleanor, >> Yes, of course you

Re: [MASSMAIL]Crawling pages but ignoring header and footer

/NUTCH-961 [2] https://issues.apache.org/jira/browse/NUTCH-585 - Original Message - From: Mark Wilson m...@sanger.ac.uk To: user@nutch.apache.org Sent: Wednesday, June 3, 2015 11:43:46 AM Subject: [MASSMAIL]Crawling pages but ignoring header and footer Does anyone know

Crawling pages but ignoring header and footer

. Any ideas greatly appreciated. Thanks Regards Mark Wilson m...@sanger.ac.uk signature.asc Description: Message signed with OpenPGP using GPGMail

[ccp4bb] Segmentation fault in Pirate cmakereference

Dear CCP4 community, I am running the make Pirate reference file utility through CCP4i using downloaded coordinates and data. It consistently fails with: #CCP4I TERMINATION STATUS 0 child killed: segmentation violation #CCP4I TERMINATION TIME 26 May 2015 17:41:21 #CCP4I MESSAGE Task failed No

parsing pages but removing headers and footers

Hi everyone. I wonder if anyone can help me. I am crawling our site with nutch 1.9, and would like to be able to parse the pages but not the headers, navbar and footer. The reason for this is because when you post it to Solr, the content field starts with the same text for all pages, and if

GSE UK Annual Conference - Large Systems Stream -- Call For Papers

All, We are beginning the process to build the agenda for the LSG stream of the next GSE UK Conference. This event will be held on November 3 4 at Whittlebury Hall in Northamptonshire. The LSG stream will comprise 10 sessions across the 2 days (5 x 60 minute sessions and 5 x 45 minute

Re: [CLucene-dev] Beginner not getting any hits on search

' index (it had no terms); -- with the change suggested above -- the index was populated with 3 terms (nixon, obama, clinton); and your query returned 1 hit in Document ID 0. Hope this helps. Regards Celto On Mon, Mar 23, 2015 at 1:24 AM, Mark Wilson mwilson1...@gmail.com wrote: I'm

[CLucene-dev] Beginner not getting any hits on search

I'm not sure what I'm doing wrong. I can see my fields in the index file, but my query/search returns no hits. Here is my index creation code: lucene::analysis::SimpleAnalyzer* analyzer; int main(int argc, char** argv) { analyzer = new lucene::analysis::SimpleAnalyzer(); Directory*

[Bug 1257186] Re: memory leakage messages (no talloc stackframe)

Confirmed in Ubuntu 14.04.02 LTS after recent upgrade from Ubuntu 12.04 LTS. -- You received this bug notification because you are a member of Ubuntu Server Team, which is subscribed to samba in Ubuntu. https://bugs.launchpad.net/bugs/1257186 Title: memory leakage messages (no talloc

[Bug 1257186] Re: memory leakage messages (no talloc stackframe)

Confirmed in Ubuntu 14.04.02 LTS after recent upgrade from Ubuntu 12.04 LTS. -- You received this bug notification because you are a member of Ubuntu Bugs, which is subscribed to Ubuntu. https://bugs.launchpad.net/bugs/1257186 Title: memory leakage messages (no talloc stackframe) To manage

Re: [LegacyUG] Any Way to Make a Family File Read-Only?

* Using WIndows Explorer, locate the family data file that you want to protect. * Right-click on the file and select 'Properties. * Select the 'General' tab and place a checkmark on the Read-Only attribute located at the bottom. --- On 13 Mar 2015 11:46 AM, GeoPappas wrote: Is there any way to

[LegacyUG] GeoExtraCodes-Alt.txt broken

:\Program Files\Legacy8\_AppData\Misc\GeoExtraCodes-Alt.txt The modifications that I made to the GeoExtraCodes-Alt.txt file were USA|111| County United States|111| County with no asterisks in front, and had no success. I appreciate any help you have to offer! Mark Wilson

[LegacyUG] GeoExtraCodes-Alt.txt FINAL FIX

such as why buttered bread always falls butter-side down! LOL! Thanks for listening! Mark Wilson --- On 12 Mar 2015 2:06 PM, Mark Wilson wrote: No, Brian, the family data file, family data file backups, and media file backups are in the directory C:\Program Files\Legacy8\_MyDocs\Wilson

Re: [LegacyUG] Re: GeoExtraCodes-Alt.txt FIXED

to properly now, and life's great! I probably wouldn't have thought to check the registry key had you not mentioned the quirks of Windows file/folder behavior. Thank you for your help! Mark Wilson --- On 12 Mar 2015 2:22 PM, Brian/Support wrote: No No No! Please get all your data, backups and Media out

Re: [LegacyUG] GeoExtraCodes-Alt.txt broken

. The customized GeoExtraCodes-Alt.txt file and the original GeoExtraCodes.txt file are both in the directory C:\Users\Mark Wilson\Documents\Legacy Family Tree\_AppData\Misc The modification to the GeoExtraCodes-Alt.txt file is USA|111| County United States|111| County Thank

[LegacyUG] Re: GeoExtraCodes-Alt.txt FIXED

Shortly after sending my last message, I found the fix to the 'GeoExtraCodes-Alt.txt' problem. Why it works, I have no idea! I placed the customized file in the directory C:\Users\Mark Wilson\My Podcasts\Legacy Family Tree\_AppData\Misc and everything works as it should! Why does

Reminder - Next GSE UK ESWG Meeting

Ladies and Gentlemen, A gentle reminder that the next meeting of the GSE (UK) Enterprise Security Working Group will take place on Thursday 5th February at CA's Office in Ditton Park, Datchet, Slough. To register, please go to http://www.racf.gse.org.uk/content/content_events.php and click on

[Bug 1399283] [NEW] openvswitch-datapath-dkms 1.4.6-0ubuntu1.12.04.4: openvswitch kernel module failed to build

Public bug reported: don't know! happened during update ProblemType: Package DistroRelease: Ubuntu 12.04 Package: openvswitch-datapath-dkms 1.4.6-0ubuntu1.12.04.4 ProcVersionSignature: Ubuntu 3.13.0-40.69~precise1-generic 3.13.11.10 Uname: Linux 3.13.0-40-generic i686 ApportVersion:

[Bug 1399283] [NEW] openvswitch-datapath-dkms 1.4.6-0ubuntu1.12.04.4: openvswitch kernel module failed to build

Public bug reported: don't know! happened during update ProblemType: Package DistroRelease: Ubuntu 12.04 Package: openvswitch-datapath-dkms 1.4.6-0ubuntu1.12.04.4 ProcVersionSignature: Ubuntu 3.13.0-40.69~precise1-generic 3.13.11.10 Uname: Linux 3.13.0-40-generic i686 ApportVersion:

Re: [ccp4bb] Strategies to bring out over-expressed protein from inclusion bodies to soluble fraction!!!

Hi Ivan, We've had good luck with the addition of chloramphenicol ~1 hour prior to harvest, as described in: Carrió, M. M., and Villaverde, A. (2001) Protein aggregation as bacterial inclusion bodies is reversible. FEBS Lett. 489, 29­33. We usually combine this with lower temperature growth (20

Re: [ccp4bb] Strategies to bring out over-expressed protein from inclusion bodies to soluble fraction!!!

...@unl.edu On 10/17/14 10:05 AM, Gloria Borgstahl gborgst...@gmail.com wrote: Thanks Mark, this is a good tip what would you use for Rosetta cells though? Tetracyclin? On Fri, Oct 17, 2014 at 8:31 AM, Mark Wilson mwilso...@unl.edu wrote: Hi Ivan, We've had good luck with the addition

[Bug 1370055] [NEW] virtualbox-guest-dkms 4.1.12-dfsg-2ubuntu0.6: virtualbox-guest kernel module failed to build

Public bug reported: virtualbox-guest-dkms 4.1.12-dfsg-2ubuntu0.6: virtualbox-guest kernel module failed to build ProblemType: Package DistroRelease: Ubuntu 12.04 Package: virtualbox-guest-dkms 4.1.12-dfsg-2ubuntu0.6 ProcVersionSignature: Ubuntu 3.13.0-36.63~precise1-generic 3.13.11.6 Uname:

Re: [ccp4bb] correlated alternate confs - validation?

As an additional comment on the issue of spatially correlated disorder, SHELX (and possibly other programs) has an elegant way of handling bump generation between disordered atoms. SHELX will only generate a bump if two atoms are in van der Waals conflict AND their occupancies sum to 1.1 or

Re: [ccp4bb] PDB passes 100,000 structure milestone

Hi Tim, I agree with everything you've said about the importance of validation, but aren't we really talking about something different here? Users of structural information should of course be keeping a careful eye on validation reports. On the other hand, what possible reason is there for the

Re: [ccp4bb] PDB passes 100,000 structure milestone

, but I think it is a good think it is not the PDB. It is a form of separation of powers. Best, Tim On 05/14/2014 06:47 PM, Mark Wilson wrote: Hi Tim, I agree with everything you've said about the importance of validation, but aren't we really talking about something different here? Users

Re: [ccp4bb] PDB passes 100,000 structure milestone

University of Nebraska N118 Beadle Center 1901 Vine Street Lincoln, NE 68588 (402) 472-3626 mwilso...@unl.edu On 5/14/14 12:41 PM, Nat Echols nathaniel.ech...@gmail.com wrote: On Wed, May 14, 2014 at 10:26 AM, Mark Wilson mwilso...@unl.edu wrote: Getting to Eric's point about an impasse

[lug:18163] Re: Which Distro to Choose...

on ubuntu software center look for powertop, ibam, and laptop mode tools. install them and reboot . report back and let us know results. On Thursday, November 15, 2012 4:04:17 AM UTC-5, Mkdir wrote: Hello EveryOne Recently i purchased Dell 7520 with 3rd gen I5 processor.I had installed

[lug:18163] Re: Laptop install issue...

you could download the driver on another pc with internet access and just copy it to a usb flash drive. or install linux to a usb flash drive using unetbootln and then copy the driver alsol On Tuesday, August 27, 2013 1:28:14 PM UTC-4, bluesf...@gmail.com wrote: My laptop's ethernet port

[nupic-discuss] Predicting multiple columns

Hi Going on from my previous question about financial data, I am modelling financial data using hourly candles (open, high, low, close). I am predicting open using open, high and low. What I would really like to try is to use only high and low and input and I would like to predict BOTH high and

Re: [nupic-discuss] Hackathon in Europe?

+1 for a Hackathon in Europe. I'm in London so here would be nice, although I haven't visited Vienna in ages! :) Mark On 16 April 2014 14:02, Ali Syed ali.dat...@gmail.com wrote: Hi All, I'm working with Matt to organise a hackathon in London, UK. If you have ideas or want to support

[LegacyUG] build 412

Thank you for adding back the Spouse/Children and Siblings Lists in Pedigree view! Mark Wilson Legacy User Group guidelines: http://www.LegacyFamilyTree.com/Etiquette.asp Archived messages after Nov. 21 2009: http://www.mail-archive.com/legacyusergroup@legacyusers.com/ Archived messages from

UK -- Next GSE Large Systems Working Group - Call for Papers

Systems focused. Would anyone be interested in presenting? If so, would you let me have a few words by way of an abstract please. Kind Regards Mark Wilson GSE Large Systems Chairman __ This message was delivered by EPA Hosted

GSE (UK) Enterprise Security Working Group

New Year. Regards Jamie Pease Mark Wilson __ This message was delivered by EPA Hosted Exchange and has been certified virus-free. For more information please visit http://www.epacloud.com/exchange

[nupic-discuss] Yet another amazing tool to try: OpenGrok

Shame about the name! You might have to call it The NuPIC Code Explorer. I have been using this wonderful tool at work. In particular I find it very useful where things aren't very well documented as it allows you to easily explore the code and associated libraries. The other thing it is very

Re: [nupic-discuss] NuPIC API docs (first pass with doxygen)

http://issues.numenta.org:/classnupic_1_1research_1_1_t_p_1_1_t_p.html doesn't work for me :( On 5 December 2013 23:41, Matthew Taylor m...@numenta.org wrote: I've been working all day with Doxygen and the temporal pooler (py/nupic/research/TP.py), and I've got some initial docs[1].

Re: [nupic-discuss] [nupic-dev] How to call TP/SP.reset() from OPF?

version of your master. You need to pull numenta/master to your local repo and build it. Check you did it correctly please.? On Sun, Nov 24, 2013 at 6:23 PM, Mark Wilson nu...@omzig.co.nz wrote: I wanted to have a play too but I'm getting RuntimeError: Spatial pooler not yet implemented in C

Re: [nupic-discuss] [nupic-dev] How to call TP/SP.reset() from OPF?

I wanted to have a play too but I'm getting RuntimeError: Spatial pooler not yet implemented in C++. I've done a git pull in my nupic repo and a cleanbuild and this hasn't helped. Any ideas? On 17 November 2013 01:57, Chetan Surpur cheta...@gmail.com wrote: Good to hear that. I've merged in

Re: [nupic-dev] Blogging about NuPIC

Good luck with the blog! I have a suggestion for a blog series: Understanding and Using Nupic It would be aimed at people who have an interest and not much else! Even though I am an experienced Linux SA and Python hacker I am struggling to get up to speed. The area is vast and the documentation

Re: [nupic-dev] Community-managed VM for NuPIC

I'm happy to seed if I can get a copy. Can someone post up the mega url? Hopefully that will match the torrent checksum. On 9 November 2013 13:39, Hannu Kettinen hannk...@gmail.com wrote: It is highly unstable. I am downloading at 80KB/s to 8MB/s i guess it is the fact that the servers are in

Re: [nupic-dev] Community-managed VM for NuPIC

, I am at 17% with MEGA DL on a 100mbit connection in EMEA. Let me get it down, then I will seed or create a new torrent and seed that so we get this going. -Hannu On Sat, Nov 9, 2013 at 2:59 PM, Mark Wilson nu...@omzig.co.nz wrote: I'm happy to seed if I can get a copy. Can someone post up

Re: [nupic-dev] Community-managed VM for NuPIC

Mega are mega slow. I traced the download and it's only coming from Luxembourg! On 9 November 2013 14:30, Mark Wilson nu...@omzig.co.nz wrote: I finally found the link - it was in this thread. Oops! I'm in London on a 130Mbit, but only getting about 3Mb from Mega. I hope the eventual file

Re: [nupic-dev] Community-managed VM for NuPIC

for that! :) 2013/11/9 Mark Wilson nu...@omzig.co.nz: Mega are mega slow. I traced the download and it's only coming from Luxembourg! -- SY Sergey V. Cleftsow ___ nupic mailing list nupic@lists.numenta.org http://lists.numenta.org/mailman/listinfo

Re: [exim] Correct permissions when delivering in maildir_format

I found the answer. The best solution for me was to make use of the multiple user mailboxes (Section 41 of the Exim Specification) Add the following entry in /etc/aliases of: #/etc/aliases jill:fred-jillsmails fred-jullsmails: fred-jillsmails And in the exim configuration

[exim] Correct permissions when delivering in maildir_format

Hi, I want to deliver mail that comes in addressed to user jill into a Maildir folder in freds Maildir structure but have not set the permissions correctly. I wonder what the most appropriate permissions should be (to keep freds privacy):- The setup is address_directory transport, with

UK: Large Systems Working Group @ GSE Conference 2013

Large Systems Working Group @ GSE Conference 2013 This year's conference promises to be bigger and better than ever. The LSG stream includes presentations by Bob Rogers IBM Distinguished engineer (retired), Marna Walle (IBM) and our very own Roger Fowler and Geoff Rousell as well as Ed Jaffe

UK GSE Conference 2013 - Registration is open

All, I am pleased to announce that the agenda for the Large Systems Stream at this years GSE conference is now finalised. Details can be found here: http://tyc.gse.org.uk/gse/presentation/view-all Registration is open and details can be found here:

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