Re: [ccp4bb] protein crystallography universe

pxuniverse - so you want to start out by excluding the nucleic acids universe? ;) Also, I note that it ends in .com. Will this be a commercial site? Will you be selling ads or some such? Cheers, - === You can't possibly be a

[ccp4bb] Atom labels in PDB file for Refmac

Dear all, This question may have been asked before, but I cannot find an answer in the recent ccp4bb archives - sorry! I am using an SeMet-protein data set as my native protein, since it diffracted to high resolution and should give all the information I need. I would therefore like to

Re: [ccp4bb] Crystal growing in one direction...need suggestions

Hi I also like to try the Hampton additive kit or the easier version; 90% current condition (at 1.1x) and 10% of any screen you can lay your hands on (ie hampton 1 2). Sitting verse hanging drops can change crystal size. Also in situ limited proteolysis, crazy but it works see for more info

Re: [ccp4bb] Atom labels in PDB file for Refmac

Hi, - MET residues should become MSE (i.e. Se-MET instead of S-Met) - ' SD ' atom name should become 'SE ' - remove the columns 77-80 - run PDBSET to let it re-generate columns 77-80 again. So something simple minded like % grep ^CRYST1 your.pdb tmp.pdb % egrep ^ATOM|^HETATM

Re: [ccp4bb] protein crystallography universe

Does this mean that you are now King of the protein crystallography universe? I am confident that you will be a kind and benevolent ruler, Diana On Oct 14, 2008, at 2:47 PM, Paul Swepston wrote: Folks: While attending the ICSG meeting in Oxford last month it struck me that there might

[ccp4bb] calc SF from map for MR - PHASER

Hi I would like to convert a map into SFs to use for molecular replacement. Read on if you think you can help. I have a 3.7A MAD data set and a few 30% similar homology models for crystal in i222 space group. The combination of the two pieces of information generated solvent flattened phases

Re: [ccp4bb] calc SF from map for MR - PHASER

Heidi Schubert wrote: Hi I would like to convert a map into SFs to use for molecular replacement. Read on if you think you can help. I have a 3.7A MAD data set and a few 30% similar homology models for crystal in i222 space group. The combination of the two pieces of information generated

Re: [ccp4bb] Crystal growing in one direction...need suggestions

Hi, you could also try microseed matrix screening in order to find new crystallization conditions. - Jeroen - shivesh kumar wrote: Dear all, I have crystallized a protein in MPD which is thin and growing in one direction only. I have tried ethonal, DMSO, Proline, Glucose, Urea, Sucrose,

[ccp4bb] Crystal growing in one direction...need suggestions

Dear all, I have crystallized a protein in MPD which is thin and growing in one direction only. I have tried ethonal, DMSO, Proline, Glucose, Urea, Sucrose, Detergent kit from Hampton and lot more alongwith temperature variation. Need suggestions regarding improvement of xtal quality, THICKNESS.