Dear Gourab,
Sorry for the delayed response, I missed your message earlier.
Can you grow the protein crystals easily (do you have lots of them)?
Is your ligand soluble in PEG, MPD, or Solutol?
Often, you can exchange the crystal solution without destroying the
crystal. But, you have to
On 22/06/2021 06:47, Jiang Xu wrote:
I have a problem building and refining an xtal structure. My protein has a N-glycosylation site and I
want to add N-acetylglucosamine and manose to my protein structure. I used Coot's carbohydrate module and
let Coot automatically build the sugar chain
Hi Jiang Xu,
(Phenixbb added as I suspect this is Phenix not coot that has caused your issue)
Did you check the "add hydrogens" button in Phenix.refine? Sometimes this can
erroneously add a second H to the ND of glycosylated Asn residues. (I assume
this is because bond distances in the input
