Long back we used Prosil solution (I think, 1% solution) for siliconization.
Mathews
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Goldman,
Adrian
Sent: Thursday, January 31, 2019 8:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Is
I fully agree with Pius.
I am very sorry other CCP users for sending one more message and filling your
mail box for some crazy guys reply.
Thanks,
Mathews
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Pius
Padayatti
Sent: Monday, October
Hi Friends,
Just checking to see whether any of you have experience with Molecular
Dimensions SpectroLight 600 Droplet DLS UV/White light imager or similar
systems.
Thank you very much,
Mathews
Hi Brad,
See below my attempts on this in 2010.
Regards,
Mathews
-Original Message-
From: Mathews, Irimpan I.
Sent: Friday, May 21, 2010 8:23 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: RE: For 24 well Netxal plates (non ccp4 question) == SUMMARY
Dear All,
Thank you for the several
Hi Christine,
Another minor point (mainly for hanging drops) not mentioned in the messages.
When you bring the crystallization tray from lower temperature to room temp.
there is a possibility for condensation and slowly droplets near the protein
drops gets absorbed into the protein drop. If
One small point:
Just make sure that you are not too off from the contents of the protein
solution. Sometimes protein solution may have a high amount of salt or things
like that and we forget to include atleast half of this concentration into the
cryo solution. This could easily crack the
Hi Jacob,
Once I had an issue with introuding CO2 into crystals and I was able to get it
bound by putting a small piece of dry ice into the well solution and keeping
the drop under CO2 pressure using nextal trays with screw cap. Please note in
this case CO2 didn't get bound by using pressure
Dear Jobi,
See if you can slowly increase the PEG concentration to 28 - 30% and that will
be a good cryo. Since BIS Tris Propane is the buffer, I think, 28% 3350 should
work fine.
If the crystals crack by going straight to 28% PEG 3350, 0.1M Bis Tris Propane
pH:6.5, 0.2M Potassium thiocyanate
Hi Katherine,
We had a case where we used saturating amounts of NaCl and precipitated the
protein to get rid of a very tight binding ligand (Structure, 11, 677-690,
2003; look at the preparation of the apoenzyme section).
Regards,
Mathews
-Original Message-
From: CCP4 bulletin board
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Mathews,
Irimpan I.
Sent: Tuesday, May 11, 2010 12:41 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] For 24 well Netxal plates (non ccp4 question)
Dear All,
Qiagen has stopped selling the 24 well Netxal
Dear All,
Qiagen has stopped selling the 24 well Netxal trays and changed to 15 well
trays (almost same price) that doesn't fit with any screens. Do you know of
some source for the 24 well screw cap trays?
Thank you very much for your help,
Mathews
: Tuesday, May 11, 2010 12:44 PM
To: Mathews, Irimpan I.
Subject: RE: [ccp4bb] For 24 well Netxal plates (non ccp4 question)
They were the only source (sigh!)
Maybe they will restart making the original product if enough of us
protests loudly enough (hopes not high).
Thierry
-Original Message
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