o: [ccp4bb] What could these crystals be?
Hi all
We have been trying with no success to crystalize a protein. Recently we got
these strange shape "crystals". They are hard and flat . Any ideas as to what
could cause this? The crystallization conditions is:0.06M Divalents 0.1M
BICINE
Hello, what is in the purification buffer?
Best wishes, Jon Cooper. jon.b.coo...@protonmail.com
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Original Message
On 13 May 2024, 15:15, 张慧欣 wrote:
> Hi all
> We have been trying with no success to crystalize a protein. Recently we got
> these
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Sent: 24 November 2023 08:59
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] What could these crystals be?
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Enviado:sexta-feira, 24 de novembro de 2023 09:00
Para: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Assunto: Re: [ccp4bb] What could these crystals be?
I wanted to thank everyone for their suggestions and ideas
: [ccp4bb] What could these crystals be?
I wanted to thank everyone for their suggestions and ideas regarding the
eyeball shaped crystals that I got at the beginning of the month.
I can confirm that these crystals do indeed contain protein and DNA which is
good news.
I have tried a number
ell.ac.uk
> tel 01235 567717
> From: CCP4 bulletin board on behalf of
> careinaedgo...@yahoo.com <02531c126adf-dmarc-requ...@jiscmail.ac.uk>
> Sent: 08 November 2023 15:00
> To: ccp4bb
> Subject: [ccp4bb] What could these crystals be?
>
> Hi all
> We have b
: CCP4 bulletin board on behalf of
careinaedgo...@yahoo.com <02531c126adf-dmarc-requ...@jiscmail.ac.uk>
Sent: 24 November 2023 08:59
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] What could these crystals be?
I wanted to thank everyone for their suggestions and ideas regarding the
e
ado:quarta-feira, 8 de novembro de 2023 17:08
Para: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Assunto: Re: [ccp4bb] What could these crystals be?
You could also run them on a gel if you don’t want to shot with canons on small
birds.
Jürgen
On Nov 8, 2023, at 11:33 AM, M T
I had the same issue before. PAS should be a decent polymer for
modification.
On Thu, Nov 9, 2023 at 7:04 PM sadik sattar wrote:
> Hi Careina,
>
> I hope this email finds you well. I wanted to share my experience with a
> similar issue I faced in the past concerning one of my proteins, and I've
Hi Careina,
I hope this email finds you well. I wanted to share my experience with a
similar issue I faced in the past concerning one of my proteins, and I've
attached a picture of my round crystals.
Despite numerous attempts with various screens and additives, I consistently
encountered
ell Oxford
> Didcot
> Oxon OX11 0FA
> United Kingdom
> Email stephen.c...@rc-harwell.ac.uk
> tel 01235 567717
> --
> *From:* CCP4 bulletin board on behalf of
> careinaedgo...@yahoo.com <02531c126adf-dmarc-requ...@jiscmail.ac.uk>
> *Sent:* 0
h
Envoyé : mercredi 8 novembre 2023 18:08
À : CCP4BB@JISCMAIL.AC.UK
Objet : Re: [ccp4bb] What could these crystals be?
You could also run them on a gel if you don’t want to shot with canons on small
birds.
Jürgen
On Nov 8, 2023, at 11:33 AM, M T wrote:
Dear Careina,
If you have easy acce
>
> Best wishes - Pascale
>
>
>
> De : CCP4 bulletin board de la part de Jurgen Bosch
>
> Envoyé : mercredi 8 novembre 2023 18:08
> À : CCP4BB@JISCMAIL.AC.UK
> Objet : Re: [ccp4bb] What could these crystals be?
>
> You could also run them on a gel if you
mercredi 8 novembre 2023 18:08
À : CCP4BB@JISCMAIL.AC.UK
Objet : Re: [ccp4bb] What could these crystals be?
You could also run them on a gel if you don’t want to shot with canons on small
birds.
Jürgen
On Nov 8, 2023, at 11:33 AM, M T wrote:
Dear Careina,
If you have easy access to mass spectro
You could also run them on a gel if you don’t want to shot with canons on small
birds.
Jürgen
> On Nov 8, 2023, at 11:33 AM, M T wrote:
>
> Dear Careina,
>
> If you have easy access to mass spectrometry, you can try to fish/rince your
> « pumpkin seeds » and send them to mass to try to
Along the lines of mass spec analysis, but probably more accessible to
many, is gel electrophoresis. Rinsing enough crystals and dissolving them
in your protein buffer should give you a sample suitable for standard
agarose and polyacrylamide gel electrophoresis protocols. I showed that
crystals I
Dear Careina,
If you have easy access to mass spectrometry, you can try to fish/rince your «
pumpkin seeds » and send them to mass to try to identify what is inside to see
if it needs optimization or not.
Best.
> Le 8 nov. 2023 à 16:03, 02531c126adf-dmarc-requ...@jiscmail.ac.uk a écrit
>
n.c...@rc-harwell.ac.uk
> tel 01235 567717
> --
> *From:* CCP4 bulletin board on behalf of
> careinaedgo...@yahoo.com <02531c126adf-dmarc-requ...@jiscmail.ac.uk>
> *Sent:* 08 November 2023 15:00
> *To:* ccp4bb
> *Subject:* [ccp4bb] What
...@rc-harwell.ac.uk
tel 01235 567717
From: CCP4 bulletin board on behalf of
careinaedgo...@yahoo.com <02531c126adf-dmarc-requ...@jiscmail.ac.uk>
Sent: 08 November 2023 15:00
To: ccp4bb
Subject: [ccp4bb] What could these crystals be? Hi allWe have been trying with
no success to crys
Dear Careina
These look like crystals indeed. Without more information, I can make some
educated guesses but the ultimate proof is in your hands :)
1. they don't have to be spherulites. Note that low-symmetry crystals can
have edge-less habits, especially P1 are notorious for that - I've had
Hi Careina,
One thing I'd mention in addition to the spot-on replies so far is that
cryoprotection strategies can influence diffraction. These look like pretty
spherulites though, so best of luck in optimization!
Andrew
On Wed, Nov 8, 2023, 10:03 AM careinaedgo...@yahoo.com <
://xray.teresebergfors.com/tutorial-3/
Good luck!
From: CCP4 bulletin board on behalf of
careinaedgo...@yahoo.com <02531c126adf-dmarc-requ...@jiscmail.ac.uk>
Sent: Wednesday, November 8, 2023 10:00 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] What could these crystals b
Have you tried crushing these up and seeding?
Jürgen
___
Jürgen Bosch, PhD, MBA
Center for Global Health & Diseases
Case Western Reserve University
Cleveland, OH 44106
https://www.linkedin.com/in/jubosch/
CEO & Co-Founder at InterRayBio, LLC
> On
2-8273
From: CCP4 bulletin board on behalf of
careinaedgo...@yahoo.com <02531c126adf-dmarc-requ...@jiscmail.ac.uk>
Sent: 08 November 2023 15:00
To: ccp4bb
Subject: [ccp4bb] What could these crystals be?
Hi all
We have been trying with no success to crystalize a protein. Rec
-harwell.ac.uk
tel 01235 567717
From: CCP4 bulletin board on behalf of
careinaedgo...@yahoo.com <02531c126adf-dmarc-requ...@jiscmail.ac.uk>
Sent: 08 November 2023 15:00
To: ccp4bb
Subject: [ccp4bb] What could these crystals be?
Hi all
We have been trying with no s
Hi allWe have been trying with no success to crystalize a protein. Recently we
got these strange shape "crystals". They are hard and flat but they do not
diffract at all. Any ideas as to what could cause this?Careina
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