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about.me/david_briggs [1]
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From: CCP4 bulletin board on behalf of Jiang Xu
Sent: Tuesday, June 22, 2021 6:47:36 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] missing covalent bond between asparagine and
N-acetylglucosamine using coot carbohydrate module
he second H is added, this breaks the refinement of the Asn-NAG bond.
>>>>
>>>> My work around is to run "Ready Set" to add hydrogens as a separate job,
>>>> and then manually inspect and correct the Asn residues as necessary before
>>>> runni
On 22/06/2021 06:47, Jiang Xu wrote:
I have a problem building and refining an xtal structure. My protein has a N-glycosylation site and I
want to add N-acetylglucosamine and manose to my protein structure. I used Coot's carbohydrate module and
let Coot automatically build the sugar chain
n, UK
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about.me/david_briggs
From: CCP4 bulletin board on behalf of Jiang Xu
Sent: Tuesday, June 22, 2021 6:47:36 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] missing covalent bond between asparagine and
N-acetylglucosamine using coot carbohydrate module and phenix.refine
External Se
Hello guys,
I have a problem building and refining an xtal structure. My protein has
a N-glycosylation site and I want to add N-acetylglucosamine and manose to
my protein structure. I used Coot's carbohydrate module and let Coot
automatically build the sugar chain to the electron density. It
