Re: [Histonet] Microwave Processing

2023-11-08 Thread Duraine, Lita R via Histonet
Hello all, I use microwave processing exclusively. First of all, are you making sure that each reagent is completely extracted before the next reagent is administered? I know the tissue cannot be completely dry, but all residue reagent should be siphoned off before the next one goes in.

Re: [Histonet] Epon Resin Embedding Question

2022-09-29 Thread Duraine, Lita R via Histonet
Hi Brian, I regularly use epon for TEM work. Even though the instructions are good for some climates , epon is particular. Here in Houston, I let my blocks cure for 5 to 7 days depending on how humid or rainy it is. When I press a fingernail into the block , if it leaves a mark I know it

Re: [Histonet] Retirement in sight!

2021-09-09 Thread Duraine, Lita R via Histonet
Congratulations Tim, I too was a graduate of the San Joaquin Delta EM School under Dr Murphy. Your story is similar to most of us. We have been able to go through doors that others only wish they could. Over the years , we often experience many accomplishments, and get to meet many