Dear everyone, I recently stained some paraffin slides of demineralized bone with DAPI. The bone (and the calcified cartilage) was so autofluorescent that the real DAPI signal of the cell nuclei was completely lost. (either it was autofluorescent, or the DAPI was not washed away properly and infiltrated the bone matrix).
I read online that it is possible to find a better (more recent) fluorochrome that emits further away for the autofluorescence emission of the tissue. Does anyone have any suggestion of a DNA fluorochrome for bone cells (in paraffin sessions?). Any other insight? The cells were permealized. As a control tissue, I used skin, and the protocol worked perfectly, only the cells were blue, but for the bone of the same animal, everything was blue. So there is a problem with the bone matrix. Thank you for your help in advance All the best -- Dr. Alida M. Bailleul Associate Professor Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences www.ivpp-avianevolution.com & Research Associate of Paleontology, Museum of the Rockies, Montana State University Google Scholar <https://scholar.google.co.jp/citations?user=Yn4PuWMAAAAJ&hl=en> - ResearchGate <https://www.researchgate.net/profile/Alida_Bailleul> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet