LOCUS       pSP70        2417 bp    DNA   circular            23-OCT-2001
DEFINITION  Cloning vector pSP70.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|38836800|
COMMENT     VNTDBDATE|245331673|
COMMENT     VNTNAME|pSP70|
COMMENT     VNTAUTHORNAME|InforMax, Inc.|
COMMENT     VNTAUTHORTEL|(301)216-0586|
COMMENT     VNTAUTHORFAX|(301)216-0087|
COMMENT     VNTAUTHOREML|informax@informaxinc.com|
COMMENT     VNTAUTHORFTP|ftp://ftp.informaxinc.com|
COMMENT     VNTAUTHORWWW|http://www.informaxinc.com|
COMMENT     VNTAUTHORAD1|6110 Executive Blvd., Suite 400|
COMMENT     VNTAUTHORAD2|North Bethesda, MD 20852|
COMMENT     VNTAUTHORAD3|U.S.A.|
COMMENT     VNTOAUTHORNAME|NCBI Entrez|
COMMENT     VNTOAUTHORTEL|(301)496-2475|
COMMENT     VNTOAUTHORFAX|(301)480-9241|
COMMENT     VNTOAUTHOREML|info@ncbi.nlm.nih.gov|
COMMENT     VNTOAUTHORWWW|http://www3.ncbi.nlm.nih.gov/Entrez/|
COMMENT     VNTOAUTHORAD1|National Center for Biotechnology Information|
COMMENT     VNTOAUTHORAD2|National Library of Medicine|
COMMENT     VNTOAUTHORAD3|Building 38A, Room 8N805|
COMMENT     VNTOAUTHORAD4|Bethesda, MD 20894, U.S.A.|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     misc_feature    1..1
                     /vntifkey="21"
                     /label=SP6_T/C_INIT
                     /note=" SP6 transcription initiation site "
     misc_feature    4..45
                     /vntifkey="21"
                     /label=POLYLINKER
                     /note=" multiple cloning sites "
     misc_feature    56..56
                     /vntifkey="21"
                     /label=T7_T/C_INIT
                     /note=" T7 transcription initiation site "
     promoter        57..73
                     /vntifkey="30"
                     /label=T7_P
                     /note=" T7 promoter "
     promoter        2401..2417
                     /vntifkey="30"
                     /label=SP6_P
                     /note=" SP6 promoter "
     CDS             complement(1090..1950)
                     /vntifkey="4"
                     /label=AP(R)
BASE COUNT      615 a       599 c       592 g       611 t 
ORIGIN
        1 gaactcgagc agctgaagct tgaattcatc gatgatatca gatctgccgg tctccctata 
       61 gtgagtcgta ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc 
      121 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 
      181 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
      241 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
      301 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
      361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
      421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
      481 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag 
      541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
      601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
      661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
      721 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
      781 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
      841 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
      901 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
      961 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     1021 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     1081 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     1141 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     1201 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     1261 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     1321 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     1381 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 
     1441 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
     1501 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
     1561 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
     1621 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
     1681 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 
     1741 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
     1801 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
     1861 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
     1921 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 
     1981 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
     2041 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 
     2101 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 
     2161 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 
     2221 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 
     2281 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga 
     2341 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg 
     2401 atttaggtga cactata 
//
