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This article may be informative: Nat Struct Biol. 2003 Mar;10(3):219-25. Extensive conformational sampling in a ternary electron transfer complex. Leys D, Basran J, Talfournier F, Sutcliffe MJ, Scrutton NS. They describe a structure of a protein-protein complex in which one large piece is disordered. > From: Song Tan <[EMAIL PROTECTED]> > Date: Tue, 3 Oct 2006 09:02:39 -0400 > To: shivesh kumar <[EMAIL PROTECTED]>, <ccp4bb@dl.ac.uk> > Subject: Re: [ccp4bb]: half of the structure missing > > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Others have made excellent comments and suggestions. I would also > offer the following precedent: > > Loris et al, JBC, 278:28252, 2003 where electron density of only half > of the MazE protein was detected, with the rest of the 82 aa protein > disordered but present in the crystal. > > The structure of the MazE/MazF complex showed that the remaining half > of MazE formed an extended chain that cofolded with the MazF partner > (Kamada et al, Mol Cell, 11:875, 2003). In the absence of MazF, this > half of MazE would not fold, consistent with the absence of defined > structure in the MazE/antibody cocrystal structure. > > Regards, > > Song > > >> Dear all, >> At present we have 2.4A resolution.the protein is supposed to have >> two domains separated by a 8-residues linker region with 3 glycines >> which we have checked with DisEMBL.So,one domain is can be >> flexible.We have done the mass spectroscopy of the protein solution >> itself and we are trying to check with the crystals also.There is no >> tag in the protein.We kept two acetate molecules and 13 water >> molecules and it has two bound calciums. >> ThanX in advance. >> shivesh > > > -- > > Dr. Song Tan > Associate Professor in Biochemistry & Molecular Biology > Center for Gene Regulation > Dept of Biochemistry & Molecular Biology > 108 Althouse Laboratory (office & lab in 3 Althouse Laboratory) > Penn State University > University Park, PA 16802 > email: [EMAIL PROTECTED] http://www.bmb.psu.edu/faculty/tan/lab/ > phone: 814-865-3355 fax: 814-863-7024
