Thanks Boaz,
They are protein crystals confirmed by spectra.
Deliang
----- Original Message -----
From: Boaz Shaanan
To: deliang
Sent: Friday, November 14, 2008 3:21 PM
Subject: Re: [ccp4bb] off-topic: soft crystals?
Hi,
You might like to check the content of those crystals by MS or running a gel
(after proper rinsing) to make sure that they're protein crystals. Based on
your description they may well be detergent crystals. I hope for you that
they're not.
Cheers,
Boaz
----- Original Message -----
From: deliang <[EMAIL PROTECTED]>
Date: Friday, November 14, 2008 23:38
Subject: [ccp4bb] off-topic: soft crystals?
To: CCP4BB@JISCMAIL.AC.UK
> Hi there,
>
> Do you ever have experience with the diffraction quality of soft
> crystals? I just harvested some. They are 30K membrane protein,
> growing 10 months, size ~0.6mm*0.2mm*0.02mm, but as soft as a
> hair, no sharp surfaces.
>
> We don't have synchrotron time in a few months and no experience
> with such crystals. Right now we need to decide whether we
> should use similar conditions for further screening or should
> change completely methods(from sitting drop to lipid cubic
> phase), and detergents. Any comments about your work with soft
> crystals are really helpful. Many thanks.
>
> Deliang
>
>
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
Phone: 972-8-647-2220 ; Fax: 646-1710
Skype: boaz.shaanan
