I would also try non-auxotrophic strains. We have recently had successful double SeCys + SeMet using BL21. Paper has just been accepted in Acta D, should be published soon.
Paula On 18 October 2010 16:05, Yogesh Gupta <yogesh.gupt...@gmail.com> wrote: > Does anyone know a commercial source of Cys-auxotroph strain of E. coli to > prepare a doubly (Se-Cys + Se-Met) labeled protein? I would appreciate if > you can share your experience if you have labeled your protein in this way > to improve Se signal for phasing. > > Thanks, > -Yogesh > > -- > -------------------------------------------------- > Yogesh K. Gupta, Ph.D. > Dept of Structural & Chemical Biology > Mount Sinai School of Medicine > Icahn Medical Institute > 1 Gustave L. Levy Place, Box 1677 > New York, NY, USA 10029 > > Tel:+1 212-659-8639 > -------------------------------------------------- >
