We are still waiting for proofs, so it might be a few weeks before its published. If anyone is interested, please email me requesting a preview version.
Paula PS: Francois, we did cite and used your work as starting point. On 20 October 2010 10:39, francois....@cbs.cnrs.fr <francois....@cbs.cnrs.fr > wrote: > Hi Paula > > Also our publication: http://www.ncbi.nlm.nih.gov/pubmed/14604526 > We can provide you the Cys-auxotroph strain of E. coli > > Francois > > > =============== Francois Hoh, PhD ================ > CBS Phone : (33) 4 67 41 77 06 > 29 rue de Navacelles Fax : (33) 4 67 41 79 13 > 34090 MONTPELLIER francois....@cbs.cnrs.fr > France > www.cbs.cnrs.fr > > > > > I would also try non-auxotrophic strains. We have recently had successful > > double SeCys + SeMet using BL21. Paper has just been accepted in Acta D, > > should be published soon. > > > > Paula > > > > On 18 October 2010 16:05, Yogesh Gupta <yogesh.gupt...@gmail.com> wrote: > > > >> Does anyone know a commercial source of Cys-auxotroph strain of E. coli > >> to > >> prepare a doubly (Se-Cys + Se-Met) labeled protein? I would appreciate > >> if > >> you can share your experience if you have labeled your protein in this > >> way > >> to improve Se signal for phasing. > >> > >> Thanks, > >> -Yogesh > >> > >> -- > >> -------------------------------------------------- > >> Yogesh K. Gupta, Ph.D. > >> Dept of Structural & Chemical Biology > >> Mount Sinai School of Medicine > >> Icahn Medical Institute > >> 1 Gustave L. Levy Place, Box 1677 > >> New York, NY, USA 10029 > >> > >> Tel:+1 212-659-8639 > >> -------------------------------------------------- > >> > > > > > > > > > >
