Any idea where then phases came from? BR -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Thomas Juettemann Sent: Wednesday, February 09, 2011 12:16 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] First images of proteins and viruses caught with an X-ray laser
Thank you for clarifying this James. Those details are indeed often lost/misinterpreted when the paper is discussed in journal club, so your comment was especially helpful. Best wishes, Thomas On Wed, Feb 9, 2011 at 20:38, James Holton <jmhol...@lbl.gov> wrote: > > As one of the people involved (I'm author #74 out of 88 on PMID > 21293373), I can tell you that about half of the three million > snapshots were blank, but we wanted to be honest about the number that > were collected, as well as the "minimum" number that were needed to > get a useful data set. The blank images were on purpose, since the > nanocrystals were diluted so that there would be relatively few > double-hits. As many of you know, multiple lattices crash autoindexing algorithms! > > Whether or not a blank image or a failed autoindexing run qualifies as > "conforming to our existing model" or not I suppose is a matter of > semantics. But yes, I suppose some details do get lost between the > actual work and the press release! > > In case anyone wants to look at the data, it has been deposited in the > PDB under 3PCQ, and the detailed processing methods published under PMID: > 20389587. > > -James Holton > MAD Scientist > > On 2/9/2011 10:38 AM, Thomas Juettemann wrote: >> >> http://www.nanowerk.com/news/newsid=20045.php >> >> http://home.slac.stanford.edu/pressreleases/2011/20110202.htm >> >> I think it is pretty exciting, although they only take the few >> datasets that conform to their existing model: >> >> "The team combined 10,000 of the three million snapshots they took to >> come up with a good match for the known molecular structure of >> Photosystem I." > >
