Based on Peter's suggestion: yes: the SUMO protease does an efficient job for removal of the tag.
Lucigen (www.lucigen.com) does sell new generation of cloning vectors (IPTG and Rhamnose inducible) with SUMO tag as well as SUMO protease included in their kit. The novel Expressioneering Technology offers very easy cloning and protein expression tools. Thanks and Good Luck. -Saurabh On Thu, Feb 24, 2011 at 12:15 PM, Peter Hsu <hsuu...@u.washington.edu>wrote: > I haven't used it personally, but I've heard some people engineering a his > SUMO tag on to the protein and then use SUMO protease to cleave it off. The > protease apparently makes very clean cuts and no extra residues are left. > > Best of luck, > Peter > --
