Hi i also faced this type of problem with my protein. i used additive screen for getting some thick needles and then with that condition i used seeding approach to get thick single crystal. try to use micro seeding with this . i used micro dilution or streak seeding for this. for seeding try to get that condition in which crystals come late......try to play with precipitant concentration for this .......
regards 2011/4/26 xinghua qin <xtal...@gmail.com> > hi,everyone, > > Sorry for the off-topic question.I got two many crystals in the drop with > long thin needle shape and crossed , after optimization, no big change > appeared, and I got only a few needle clusters in the drop at most times. > which one is better for next optimization step and suitale to be the > "working solution"? In my opinion, the latter one has fewer nucleus, but the > former one has better shape, did I got the point? > > Best wishes > > Xinghua Qin > > -- > Xinghua Qin > Room 4022, Center for Life Sciences, > China Agricultural University, > No.2, Yuan Ming Yuan West Road, Haidian District， > Beijing，China，100193 > Tel: +86-10-62732672 > > -- Vandana Kukshal Postdoc Fellow structure biology group international Center For Genetic Engineering and Biotechnolgy India
