Thanks to all , The protein storage buffer has MgCl2, As per your observaitions, it might be this combination gave me the hexagon crystal,
but I have decided to put them in beam this week, Will report as soon as i get a few images. thanks once again to all, S.Jayashankar Research Student Institute for Biophysical Chemistry Hannover Medical School Germany. On Mon, May 30, 2011 at 10:37 AM, <herman.schreu...@sanofi-aventis.com>wrote: > Dear Jay, > They may all be salt. However, compared to the effort of producing the > protein and setting up the crystallization trials, testing the crystals in > an X-ray beam is trivial. In your place, I would test them all, either on a > home source or at the synchrotron. I have had one case when there were > protein crystals and ammonium sulfate crystals in the same drop. You do not > want to miss those protein crystals! > > Best, > Herman > > ------------------------------ > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of * > Jayashankar > *Sent:* Sunday, May 29, 2011 11:18 PM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] salt or protein... > > Dear Friends , > > I need to know whether phosphate can form hexagon shaped crystals. > In one particular condition i have 4 different pattern , 3 seems to me as > salt crystals, except for the one... > please find the attachment for the reference.. > > Thanks in advance > Jay > Institute for Biophysical Chemistry > Hannover Medical School > Germany. > >