Sorry if I sound rather pessimistic, but it's extremely likely these crystals are all salt. I've seen this morphology of Phosphate before, and it forms both the X-shaped crystals (plates with sides un-filled) and poorly shaped fat hexagonal blocks.
This condition is also a dead giveaway - lots of phosphate (and some Mg from protein) together with high organic (which is going to make phosphate rather insoluble). Artem On Mon, May 30, 2011 at 3:46 AM, Jayashankar <s.jayashan...@gmail.com>wrote: > Thanks to all , > > The protein storage buffer has MgCl2, > As per your observaitions, it might be this combination gave me the hexagon > crystal, > > but I have decided to put them in beam this week, > Will report as soon as i get a few images. > > thanks once again to all, > > > S.Jayashankar > Research Student > Institute for Biophysical Chemistry > Hannover Medical School > Germany. > > > On Mon, May 30, 2011 at 10:37 AM, > <herman.schreu...@sanofi-aventis.com>wrote: > >> Dear Jay, >> They may all be salt. However, compared to the effort of producing the >> protein and setting up the crystallization trials, testing the crystals in >> an X-ray beam is trivial. In your place, I would test them all, either on a >> home source or at the synchrotron. I have had one case when there were >> protein crystals and ammonium sulfate crystals in the same drop. You do not >> want to miss those protein crystals! >> >> Best, >> Herman >> >> ------------------------------ >> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of >> *Jayashankar >> *Sent:* Sunday, May 29, 2011 11:18 PM >> *To:* CCP4BB@JISCMAIL.AC.UK >> *Subject:* [ccp4bb] salt or protein... >> >> Dear Friends , >> >> I need to know whether phosphate can form hexagon shaped crystals. >> In one particular condition i have 4 different pattern , 3 seems to me as >> salt crystals, except for the one... >> please find the attachment for the reference.. >> >> Thanks in advance >> Jay >> Institute for Biophysical Chemistry >> Hannover Medical School >> Germany. >> >> >
