CD spec with Pelletier is an option too Jürgen
...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Oct 26, 2011, at 19:08, Reginald McNulty <rmcnu...@uci.edu> wrote: > We are trying to determine Tm value of rather unstable proteins with a Tm in > the mid 20 C range using DSF/thermofluor. Our Stratagene thermocycler has a > low temp limit of 25 C (Peltier). I called the company and they said it's a > 'hardware limit' that cannot be changed. > > 1) Has anyone been able to 'hotwire' the MX3000/3005 to go below this limit? > > 2) Are there other thermofluor machines that allow lower starting > temperatures (say 4, 10 or 15 C)? > > Best regards, > -Reggie > >
