Hi,
I'm sure there are proteins that were crystallized at low pH but I can't
remember which. The best thing is to go to the BMCD database:
http://xpdb.nist.gov:8060/BMCD4/index.faces
and query it with the key pH (look into advanced search).
Cheers,
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: bshaa...@bgu.ac.il
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
________________________________________
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Sam Arnosti
[meisam.nosr...@gmail.com]
Sent: Monday, November 07, 2011 7:19 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystalization in low PH
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the low PH and compare the differences between the
crystals in regular PH and low PH.
I was wondering how people set up the boxes in low PH, as usual buffers are
mostly less acidic.
Regards
Sam
