Tendamistat (1OK0) was crystallized at pH 1.3 and diffracted to 0.93A. George
On Mon, Nov 07, 2011 at 05:19:29AM +0000, Sam Arnosti wrote: > Hi everyone > > I have a protein that is extraordinarily stable at PH=3.0 or even 2.0. > > I want to crystallize it in the low PH and compare the differences between > the crystals in regular PH and low PH. > > I was wondering how people set up the boxes in low PH, as usual buffers are > mostly less acidic. > > Regards > > Sam > -- Prof. George M. Sheldrick FRS Dept. Structural Chemistry, University of Goettingen, Tammannstr. 4, D37077 Goettingen, Germany Tel. +49-551-39-3021 or -3068 Fax. +49-551-39-22582
