Glutaraldehyde works best at low pH
On Mon, Nov 7, 2011 at 8:40 AM, Ed Pozharski <epozh...@umaryland.edu> wrote: > On Mon, 2011-11-07 at 05:19 +0000, Sam Arnosti wrote: >> Hi everyone >> >> I have a protein that is extraordinarily stable at PH=3.0 or even 2.0. >> >> I want to crystallize it in the low PH and compare the differences between >> the crystals in regular PH and low PH. >> >> I was wondering how people set up the boxes in low PH, as usual buffers are >> mostly less acidic. >> >> Regards >> >> Sam > > Not clear if you already have crystals at "regular pH", but if you do, > you may consider direct transfer to lower pH. Of course, crystals may > dissolve, which you could possibly prevent by cross-linking with > glutaraldehyde. Three caveats: > a) If lattice is incompatible with lower pH, even with cross-linking the > resolution may sink to essentially useless levels > b) I have no idea if the cross-linking will not be disrupted at really > low pH, perhaps someone else can comment on that > c) the 3rd reviewer can always say that lattice forces could have > prevented a conformational change. But same goes for direct > crystallization at low pH (but caries less weight). > > -- > "I'd jump in myself, if I weren't so good at whistling." > Julian, King of Lemurs >
