Dear All, I have read a recent paper by Salgado et al about using non-auxotrophic E.coli to incorporate SeCys into recombinant protein for phasing purposes. Does anyone have a source for Selenocysteine? I have also seen a paper by Schaefer et al which uses nitric acid treated elemental Se for a sulfur surrogate to generate Se-labeled protein. Has anyone else tried this? My proteins are rich in Cys and tend to lack Met so the prospect of labeling cys is very attractive.
Thanks, Linda ________________________ Linda Olson, PhD Research Scientist II Dept Biochemistry Medical College of Wisconsin 8701 Watertown Plank Rd Milwaukee, WI 53226 phone: 414-955-8545 fax: 414-456-6510 _________________________
