You might try mercury soaks instead. Might give you 15 minute answers to your questions.
HNY! Ho Sent from my iPad On Dec 26, 2012, at 4:00 PM, CCP4BB automatic digest system <lists...@jiscmail.ac.uk> wrote: > There is 1 message totaling 34 lines in this issue. > > Topics of the day: > > 1. SeCys usage for SAD > > ---------------------------------------------------------------------- > > Date: Wed, 26 Dec 2012 12:33:02 -0600 > From: Linda Olson <lol...@mcw.edu> > Subject: SeCys usage for SAD > > Dear All, > > I have read a recent paper by Salgado et al about using non-auxotrophic > E.coli to incorporate SeCys into recombinant protein for phasing purposes. > Does anyone have a source for Selenocysteine? I have also seen a paper by > Schaefer et al which uses nitric acid treated elemental Se for a sulfur > surrogate to generate Se-labeled protein. Has anyone else tried this? My > proteins are rich in Cys and tend to lack Met so the prospect of labeling cys > is very attractive. > > Thanks, > > Linda > ________________________ > Linda Olson, PhD > Research Scientist II > Dept Biochemistry > Medical College of Wisconsin > 8701 Watertown Plank Rd > Milwaukee, WI 53226 > > phone: 414-955-8545 > fax: 414-456-6510 > _________________________ > > ------------------------------ > > End of CCP4BB Digest - 24 Dec 2012 to 26 Dec 2012 (#2012-363) > *************************************************************
