Side note, Has anyone tried this in a non-E. coli expression system?
Thanks, Lori Lori Horton, Ph.D. Koehler Lab lori.b.hor...@uth.tmc.edu<mailto:lori.b.hor...@uth.tmc.edu><mailto:lori.b.hor...@uth.tmc.edu> 713-500-5451 ________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of A K [alek6...@gmail.com] Sent: Tuesday, February 26, 2013 4:45 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Additives to bacterial cultures to improve solubility Mark, you can try low percentage of ethanol as well, as it increases the expression of some chaperones (can't find the paper right now!). Alex On Tue, Feb 26, 2013 at 2:08 AM, Mark J van Raaij <mjvanra...@cnb.csic.es<mailto:mjvanra...@cnb.csic.es>> wrote: Dear All, We are considering trying additives to bacterial cultures to see if they improve solubility of some proteins we express: L-arginine, glycyl-glycine, sorbitol and betaine. I would be glad to receive recommendations and experiences, also if they haven't worked. Side-question: should we get cell culture-certified compounds, or would "normal", much more economical ones suffice? (i.e. are they likely to contain side-products that would affect bacterial growth?). Greetings, Mark Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616<tel:%28%2B34%29%2091%20585%204616> http://www.cnb.csic.es/~mjvanraaij<http://www.cnb.csic.es/%7Emjvanraaij> -- David J. Leibly Graduate Student, Yeates Lab Dept. Chem & Biochem Univ. of California, Los Angeles Boyer Hall 269B (310) 825-8901
