Thanks every one for the great help. I am loaded with all the useful information.
best Adriana On Tue, May 2, 2017 at 9:07 PM, Deaconescu, Alexandra < alexandra_deacone...@brown.edu> wrote: > Hi, > > ATPgS is an option, but it is slowly hydrolysable, so depending on your > enzyme, it might not be the best one to use. > > Cheers, > Alexandra > > > On Tue, May 2, 2017 at 2:56 PM, Aaron Thompson <aaron.a.thomp...@gmail.com > > wrote: > >> ATP-gamma-S is another option. There are 123 public structures >> containing this ligand (ligand ID AGS). >> >> >> Cheers, >> >> >> Aaron >> >> On Tue, May 2, 2017 at 11:42 AM, Kevin Jude <kj...@stanford.edu> wrote: >> >>> ADP-BeFx and ADP-AlFx (the latter mimics a dissociative transition >>> state). The fluoride compounds can be made by mixing BeCl2 or AlCl3 with >>> excess NaF and then used at say a 3:1 excess over ADP. Be sure to dissolve >>> the chloride compounds in a fume hood! >>> >>> Also ADP-VO4, which can be prepared as described in Goodno CC. Myosin >>> active-site trapping with vanadate ion. Meth Enzymol. 1982;85 Pt B:116–23. >>> >>> Best wishes >>> Kevin >>> >>> On Tue, May 2, 2017 at 9:04 AM, Adriana Sene <adrianasen...@gmail.com> >>> wrote: >>> >>>> Hi >>>> What are good ATP non hydrolyzable analog for crystallization? >>>> >>>> best >>>> >>>> Adriana >>>> >>> >>> >>> >>> -- >>> Kevin Jude, PhD >>> Research Specialist, Garcia Lab >>> Departments of Molecular & Cellular Physiology and Structural Biology >>> Stanford University School of Medicine >>> Beckman B177, 279 Campus Drive, Stanford CA 94305 >>> Phone: (650) 723-6431 >>> >> >> >
