Hi, you could try picking in the cold room. Provided the temperature change does not kill the crystals, this sometimes worked fine for me in similar cases. Petri
Petri Kursula ---------- Professor Department of Biomedicine University of Bergen, Norway http://www.uib.no/en/rg/petrikursula <http://www.uib.no/en/persons/Petri.Kursula> petri.kurs...@uib.no <mailto:petri.kurs...@biomed.uib.no> ---------- Group Leader, Adjunct Professor Faculty of Biochemistry and Molecular Medicine University of Oulu, Finland ---------- > On 14 Aug 2018, at 20:58, Thomas Krey <krey.tho...@mh-hannover.de> wrote: > > Dear crystallization experts, > > We have 3D protein crystals grown from a microseed matrix screening vapor > diffusion experiment in either > > 15% (v/v) Reagent alcohol > HEPES Na pH 7.5 > 0.2 M MgCl2 > > or in > > 27% Isopropanol > 0.18 M MgCl2 > 90 mM HEPES Na pH 7.5 > 10% Glycerol > > Upon opening the corresponding wells these crystals move quite a bit – > presumably due to the volatility of the alcohols. Does anyone have a good > suggestion to stabilize the swirling movements? Does anyone have experience, > whether these conditions alone can serve as cryo-protectant (i.e., do we > really have to fish, move into cryo solution and fish again)? > Any suggestion or input would be highly welcome. > > Thank you very much in advance. > > Thomas > > > Prof. Dr. Thomas Krey > Hannover Medical School > Institute of Virology > Structural Virology Group > Carl-Neuberg-Str. 1 > D-30625 Hannover > phone: +49 (0) 511 - 532 4308 > email: krey.tho...@mh-hannover.de <mailto:krey.tho...@mh-hannover.de> > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
