My only comment is that you should consider not only what is in reservoir, but also anything present in your sample buffer.
On Jul 18, 2019, at 3:02 PM, Nicola Evans <0000251ca3b4615e-dmarc-requ...@jiscmail.ac.uk<mailto:0000251ca3b4615e-dmarc-requ...@jiscmail.ac.uk>> wrote: (well conditions: 0.2 M Magnesium chloride hexahydrate, 0.1 M BIS-TRIS pH 5.5, 25% PEG 3,350). As this is an the crystal contact region the molecule isn't likely to be biologically significant. The data have been solved to 1.9Å. I have attached a screenshot of the mysterious density. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
