I would like to know how to block activated groups on Thiol-activated Sepharose 4B (after coupling a peptide). The immobilized peptide will be used later to purify/identify which antibody binds to this peptide.
For CNBr-activated sepharose I use Tris or ethanolamine to block the remaining active groups, but I cannot find anywhere what should I use for thiol-activated sepharose. I thought I could use cysteine for blocking, but I am afraid it will compete out my bound peptide. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
