I would try iodoacetamide. Sent from my iPhone
> On Feb 25, 2021, at 7:58 PM, David <david.sa...@gmail.com> wrote: > > EXTERNAL EMAIL – Use caution with any links or file attachments. > > I would like to know how to block activated groups on Thiol-activated > Sepharose 4B (after coupling a peptide). The immobilized peptide will be used > later to purify/identify which antibody binds to this peptide. > > For CNBr-activated sepharose I use Tris or ethanolamine to block the > remaining active groups, but I cannot find anywhere what should I use for > thiol-activated sepharose. > > I thought I could use cysteine for blocking, but I am afraid it will compete > out my bound peptide. > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
