GFP:H6 is a nice place to start. it doesn't have enzymatic activity, but it expresses nicely in coli and is visible to the human eye during affinity-purification on NiNTA/imidazole (and the fluorescence is interesting too). the biochemistry dept. here uses it routinely with undergrads. cheers jon
Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> Im Auftrag von Hans-Petter Hersleth Gesendet: Donnerstag, 17. Juni 2021 10:12 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] Looking for proteins for undergraduate biochemistry lab We can recommend a small flavoprotein. which we have used for different biochemistry courses. It is easy to express and purify with IEX snd GF (no tag), and in the 15 years we have used it, students always get crystals. We have published it in Biochemistry and molecular biology education: "A Research-inspired biochemistry laboratory module - combining expression, purification, crystallization, structure-solving, and characterization of a flavodoxin-like protein” https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.21218 Best Hans-Petter On 17 Jun 2021, at 00:18, P. H <duskyde...@gmail.com<mailto:duskyde...@gmail.com>> wrote: Hello All, We are looking for some candidate proteins for an undergraduate level advanced biochemistry lab. They should be expressed in bacteria, simple enough to purify and it will be nice to perform some simple characterization experiments(binding assays, enzymatic assays). Any suggestions? Thank you in advance. Prerna gupta ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ---------------------------------------------------------------------------------------------- Hans-Petter Hersleth, PhD Senior Lecturer of Biochemistry & Structural Biology University of Oslo, Department of Biosciences & Department of Chemistry Structural Redox Biochemistry - Hersleth lab http://hersleth.org/<http://www.hersleth.org/> Contact information: Department of Biosciences, University of Oslo Section for Biochemistry and Molecular Biology P.O.Box 1066 Blindern, NO-0316 Oslo, Norway Phone: +47 2285 4631 Mobile: +47 413 33 126 ----------------------------------------------------------------------------------------------- ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
