All the previously suggested proteins are awesome candidates!

Here are a few more in case you want to add some extra 'zing' to your
curriculum:

- luciferase
- magneto reactive protein(s) that can be isolated with strong magnets
- spy-tag and spycatcher (covalently reactive proteins!)
- split GFP components (react and create fluorescence where there was none)
- dextransucrase (makes gels out of sucrose)
- gelatinase (dissolves gel)
- secreted thermolysin (followed by aspartame synthesis demo)
- ruby-tag (rubredoxin) very dark brown, does not denature on gel
- DHOD converts blue 2,6-dichloroindophenol into colorless reduced form
that spontaneously reoxidizes back to blue in air

And many others :)

Artem




On Wed, Jun 16, 2021, 6:19 PM P. H <duskyde...@gmail.com> wrote:

> Hello All,
>
> We are looking for some candidate proteins for an undergraduate level
> advanced biochemistry lab. They should be expressed in bacteria, simple
> enough to purify and it will be nice to perform some simple
> characterization experiments(binding assays, enzymatic assays).
> Any suggestions?
>
> Thank you in advance.
> Prerna gupta
>
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