Hi
As total insolubility does not exist, I regularly use another method,
which is to deposit a grain of the ligand directly into the drop.
In this case, we no longer control the concentration. The goal is to
regularly freeze crystals (1 hour, 2 days, 1 week ..). Depending on the
results, it is possible to adjust the time. If after one hour, the
crystal no longer diffracts, redo the experience by freezing at 10s,
30s, 2mn ..). If a blob appears, frozen after 1 week. This technique
needs 2 things
, time and several exploitable crystals in the drop.
FH
--
________________________________________
François Hoh
Centre de Biochimie Structurale,
UMR 5048 CNRS, UMR 1054 INSERM
29, rue de navacelles
34090 Montpellier Cedex, France.
Phone: +33 467 417 706
Fax: +33 467 417 913
________________________________________
--
This message has been scanned for viruses and
dangerous content by MailScanner, and is
believed to be clean.
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list
hosted by www.jiscmail.ac.uk, terms & conditions are available at
https://www.jiscmail.ac.uk/policyandsecurity/
