Hi Samer, SAbDab is an excellent resource for sequence, structure, and affinity data for publicly available antibodies.
A quick look shows that Fab-antigen structures 3h42, 2nyy, 4ps4, 2bdn, and 2nz9 all have reported interaction affinities in the low pM range. Probably worth confirming this with the actually publications, but should be a good start! Best of luck, Danton ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of samer halabi <000030c2162795b2-dmarc-requ...@jiscmail.ac.uk> Sent: December 12, 2021 10:31 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] High-affinity binding Fab to a known epitope amino acid sequence Dear All, Sorry to be disturbing you all, however the valuable input I get each time I have an inquiry from the mailing list members, encourages me to inquire again. Do you know of (or use) a Fab with high-affinity of binding to its epitope Dear All, Sorry to be disturbing you all, however the valuable input I get each time I have an inquiry from the mailing list members, encourages me to inquire again. Do you know of (or use) a Fab with high-affinity of binding to its epitope (KD in the Picomolar pM or the Femtomolar fM range), where the amino acid sequence of the epitope is also known? I have searched the commercially available Fabs, however the epitope amino acid sequence is not disclosed to the researchers. Thank you. Best regards, Samer ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://urldefense.com/v3/__https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1__;!!D0zGoin7BXfl!r_SzHQVLjagVaP0l-m_sWsBTvTox0RmnUfVd54oBfMalXXcoKrTPQr_eN41q5INXKMwIOzWk$> ________________________________ This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
