Another recommendation: Increase the concentration of your pH buffer, if possible, for better results (possibly a larger yield). I agree that some components, especially the sugars, should not be autoclaved and should be filtered instead.
Daniel ________ Daniel M. Himmel, Ph. D. Principal, Himmel Sci Med Com, LLC E-mail: danielmhim...@gmail.com URL : https://www.HimmelSciMedCom.com On Mon, Oct 2, 2023 at 11:05 AM Artem Evdokimov <artem.evdoki...@gmail.com> wrote: > These are the ones I've used in the past (I mostly use home-made, since it > gives one more control over the process). For folks who prefer convenience, > please see below (Thermo's $146/liter, though... LOL). Also, in fairness, > at least the Millipore/Sigma offers you isotopically defined media > preparations. > > > https://formedium.com/product-category/formedium-media/escherichia-coli-media/aim-auto-induction-medium/ > https://bocascientific.com/gcm20-0500-506-detail > Overnight Express (VWR or Millipore) > Magic Media (Thermo) > > I do NOT recommend autoclaving complete formulations (like the Boca Sci. > one above) because sugars in the medium react with amino acids resulting in > Brown Evil. Look it up if you're curious... > > In recognition of the original author, references to Studier papers: > > 2005: https://pubmed.ncbi.nlm.nih.gov/15915565/ > 2014: https://pubmed.ncbi.nlm.nih.gov/24203322/ > > Artem > > - Cosmic Cats approve of this message > > > On Mon, Oct 2, 2023 at 10:15 AM Artem Evdokimov <artem.evdoki...@gmail.com> > wrote: > >> Dear Jinhui Dong >> >> You can simply make swimming-pool size amounts of it, as long as you have >> basic lab equipment and supplies including an autoclave, and access to ~8 >> chemicals. The recipe is in the Studier papers, but I am happy to share it >> with you here if you prefer. >> >> Artem >> >> - Cosmic Cats approve of this message >> >> >> On Mon, Oct 2, 2023 at 9:39 AM Dong, Jinhui <jinhui_d...@brown.edu> >> wrote: >> >>> Hi, >>> Could you please post the following message on ccp4bb? >>> >>> Autoinduction medium >>> We need autoinduction medium for protein expression in E coli. Could >>> anyone provide information on USA suppliers with trustworthy products and >>> reasonable prices? Thank you! >>> Jamse >>> Brown University >>> >>> Thanks! >>> >>> Jinhui Dong >>> >>> ------------------------------ >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >>> >> > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
