Hi Everyone, I have always done the quench 1st if I do it manually. If it is automated, then unless you have a newer instrument with onboard retrieval, you don't have a choice but to quench after the retrieval step (and may not have a choice then depending upon the system and how "open" it is).
Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 [EMAIL PROTECTED] phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Greg Dobbin Sent: Thursday, September 25, 2008 3:12 PM To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] which one first - antigen retrieval or endoperoxidase block? I'd say it doesn't matter which is 1st, except my inclination is always to do the quench step 1st because the tissues seem a little less delicate before retreival than after. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln >>> "Monfils, Paul" <[EMAIL PROTECTED]> 9/25/2008 3:26:01 PM >>> When doing immunoperoxidase staining on a tissue that requires antigen retrieval, which do you do first - the antigen retrieval or the endogenous peroxidase blocking? Or does it matter? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet