Hi, everyone
I want to do pan-keratin IHC for some five year old pap smear slides. After I remove the coverslip then I used following protocol for destaining (xylene 5 min, two time change; 3% acetic alcohol (95%) overnight; 70% Etoh for 5 min) and then I put it in PBS, blocking, Prmary Ab, Secondary Ab and DAB. Problem is that I got less or nothing staining for pan-keratin Ab (even I used different dilution of Ab). Does anyone know how to recover the antigen activity for these slides? Any suggest will be apprecaited.--hao _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet