Dear Histonetters,
I'm looking for methods to preserve intestinal tissue samples, in order
to later isolate RNA from laser microdissected cryosections.
I'm a bit worried about the intestinal enzymes (not sure if the RNA will
be preserved even at -80C for very long). I was told that RNALater might
be better, but I don't have personal experience on the stuff.
Does anybody know if RNALater-treated tissues can be later frozen and
cut to adequate cryosections?
How about antigenicity, as we would probably like to use the same
samples for immunohistochemistry as well?
Another question: is it true that OCT-embedded frozen tissues can be
used to isolate RNA (with Trizol, for example)?
I know these have been discussed in the past, but couldn't find definite
answers in the archives. Perhaps more experience has been gathered since
then!
With best regards,
Mikael Niku
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Mikael Niku, PhD, university lecturer
University of Helsinki, Division of Nutrition
URL: mikael.nikunnakki.info
- What do I think of western civilization?
I think it would be a good idea! (Gandhi)
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