Thanks that is the one I am looking for! Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: [EMAIL PROTECTED]
-----Original Message----- From: Weems, Joyce [mailto:[EMAIL PROTECTED] Sent: Mon 10/20/2008 11:16 AM To: Jo-Ann Bader, Ms.; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Baker's Fluid This was in the archives. Hope it helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax On Wed, 24 May 2000, Barry Rittman wrote: > what you need is "Mollifex" sold by BDH You shouldn't use secret mixtures, but Mollifex isn't (or isn't quite) a secret formulation. BDH made it following a publication by J. R. Baker (1941) A fluid for softening tissues embedded in paraffin wax. Quart. J. Microsc. Sci. 61:75. Bakers experiments led him to the composition: Glycerol 10 60% ethyl alcohol: 90 (parts by volume). A more recent paper is by Maria Wynnchuk (1992) in J. Histotechnol. 15(4): 321-323. She used a mixture called Horne's modification of Baker's solution: 95% ethanol 9520 ml Glycerol 320 ml Acetone 80 ml Liquid phenol 80 ml (I guess that the "liquid phenol" is an 80% solution in water, not solid phenol that has been melted by heating.) 10 litres of softening solution seems a lot to make up! I wonder how long it lasts for. Wynnchuk states that this mixture is better than Baker's original and that Mollifex, which contains a "phenol derivative" is just as good. Anything here for Messrs Sue, Grabbit and Runne? John A. Kiernan, Department of Anatomy & Cell Biology, The University of Western Ontario, LONDON, Canada N6A 5C1 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jo-Ann Bader, Ms. Sent: Monday, October 20, 2008 10:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Baker's Fluid Does anyone have the recepe fpr Baker's fluid? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: [EMAIL PROTECTED] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet