Hi Margaret Perry ,
You can put your slides in to cold solution and put in to pressure cooker then
you can set the temperature to 95 C for 12 - 15 min is fine or after reaching
to 95 C you can keep it for 6 - 8 min is good. This protocol is good for when
you use 10% Neutral buffered formaline as a fixative.
Regards,
Kamal
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