Incubate with one antibody and use DAB as the chromagen, then go back and incubate with the second antibody and use a colored chromagen such as AEC.
Paula ----- Original Message ---- From: "Edwards, R.E.." <[EMAIL PROTECTED]> To: histonet <histonet@lists.utsouthwestern.edu> Sent: Monday, November 3, 2008 8:08:46 AM Subject: [Histonet] double trouble OK chaps, which in your opinion is the best double labelling method when one is using primary antibodies raised in the same species, I am aware that Vector labs supply such a kit; many thanks.. Cheers Richard Edwards University of Leicester U.K.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet