Hopefully Chris van der Loos is looking in on this and will send the person
who needs to do double IHC. He just published a very useful method in
Journal of Histotechnology on this subject where the first antibody complex
is removed, leaving behind chromogen after a retrieval method followed by
the second antibody complex with a different chromogen. He may have a pdf
of his publication available.
Gayle M. Callis
HTL/HT/MT(ASCP)
----- Original Message -----
From: "Paula Pierce" <[EMAIL PROTECTED]>
To: "Edwards, R.E." <[EMAIL PROTECTED]>; "Histonet"
<histonet@lists.utsouthwestern.edu>
Sent: Monday, November 03, 2008 7:23 AM
Subject: Re: [Histonet] double trouble
Incubate with one antibody and use DAB as the chromagen, then go back and
incubate with the second antibody and use a colored chromagen such as AEC.
Paula
----- Original Message ----
From: "Edwards, R.E.." <[EMAIL PROTECTED]>
To: histonet <histonet@lists.utsouthwestern.edu>
Sent: Monday, November 3, 2008 8:08:46 AM
Subject: [Histonet] double trouble
OK chaps, which in your opinion is the best double labelling method when one
is using primary antibodies raised in the same species, I am aware that
Vector labs supply such a kit; many thanks..
Cheers
Richard Edwards
University of Leicester
U.K....
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